一测多评法同时测定不同产地淫羊藿提取物中的4种黄酮类成份

Simultaneous Determination of Four Flavonoids Compounds in Epimedium Extract by Quantitative Analysis of Multi-components with A Single-marker

关键词:  一测多评  淫羊藿  天然植物提取物  质量标准

Key words:  quantitative analysis of multi-components by single-marker(QAMS)  Epimedium herb  natural plant extract  quality standard

摘要:

建立一测多评( QAMS) 法同时测定淫羊藿提取物中4种黄酮类化合物的含量，并验证了该方法的准确性和可行性。采用C18色谱柱，乙腈-水为流动相，检测波长270 nm，建立朝藿定A、朝藿定B、朝藿定C与内标物淫羊藿苷的相对校正因子(RCF)，比较分析QAMS法与外标法含量测定结果的差异性。结果显示，6批不同产地和批号的淫羊藿提取物中4种黄酮类成分采用一测多评法与外标法的实测值之间无显著性差异。

Abstract:

To establish a quantitative analysis method of multi-components by single-marker (QAMS) for determination of 4 flavonoids compounds in Epimedium extract and verified the accuracy and feasibility of the method. Using C18 chromatographic column, acetonitrile-water as mobile phase, detection wavelength of 270 nm，the relative correlation factors of Epimendin A, Epimendin B, and Epimendin C were calculated and established by HPLC with icariin as internal reference substance, and the difference of content determination results between QAMS method and external standard method was analyzed. The result showed the contents of 4 flavonoids compounds in 6 batches of Epimedium extract determined by QAMS method had no significantly difference with external standard method.