猪瘟猪丹毒猪肺疫三联活疫苗抗原菌选择性显色培养基的研发和应用

Development and application of the selective chromogenic medium for antigen bacteria of Combined Classical Swine Fever-Swine Erysipelas and Pasteurella multocida Live Vaccine

关键词:  猪瘟猪丹毒猪肺疫三联活疫苗  显色培养基  显色底物  活菌计数  回收率

Key words:  Combined Classical Swine Fever-Swine Erysipelas and Pasteurella multocida live vaccine  chromogenic medium  chromogenic substrate  bacterial plate count  recovery rate

摘要:

为了研制便于检测猪瘟猪丹毒猪肺疫三联活疫苗抗原含量的选择性显色计数琼脂（以下简称猪三联显色培养基），以代替传统的马丁琼脂。本研究通过平行对比干粉马丁琼脂、新鲜马丁琼脂和猪三联显色培养基的理化特性和生长特性，并通过活菌计数的方法，平行对比猪丹毒单价苗、猪肺疫单价苗和猪三联苗的抗原含量。结果表明，猪三联显色培养基在活菌计数的准确性和重复性上均能与干粉马丁琼脂保持一致，而且两种菌的菌落回收率均能达到0.9以上。因此，本研究中的猪三联显色培养基可以代替马丁琼脂作为检测猪丹毒和猪肺疫抗原菌含量的计数琼脂，在猪三联活疫苗生产过程中监测疫苗质量。

Abstract:

In order to replace traditional Martin agar, a selective chromogenic counting agar (hereinafter referred to as Swine triplex vaccine chromogenic medium) was developed for detecting antigen content of Combined Classical Swine Fever, Swine Erysipelas and Pasteurella multocida live vaccine Swine Fever-Swine Erysipelas and Pasteurella multocida live triplex live vaccine. In this study, the physical characteristics and growth characteristics of dry powder Martin agar, fresh Martin agar and Swine triplex vaccine chromogenic medium were compared in parallel, and the antigen content of Swine Erysipelas live vaccine, , Swine Pasteurella multocida live vaccine and Combined Classical Swine Fever, Swine Erysipelas and Pasteurella multocida live vaccine Swine triplex vaccine were compared in parallel by bacteria plate counting. The study results showed that the accuracy and repeatability of the bacteria plate count of Swine triplex vaccine chromogenic medium were consistent with that of dry powder Martin agar, and the colony recovery rate of both bacteria was above 0.9. Therefore, the Swine triplex vaccine chromogenic Medium in this study can replace Martin Agar as a counting agar to detect the content of antigen bacteria of Swine Erysipelas and Swine Pasteurella multocida, and monitor the quality of vaccine in the production process of Combined Classical Swine Fever, Swine Erysipelas and Pasteurella multocida live vaccine Swine triplex live vaccine.