高效液相色谱法测定柴辛注射液中有效组分含量的研究

Study on Simultaneously Determination Method of Methyleugenol and Asarinin in Chaixin Injection by High Performance Liquid Chromatography

关键词:  柴辛注射液  甲基丁香酚  细辛脂素  高效液相色谱法  核壳填料

Key words:  Chaixin Injection  Methyleugenol  Asarinin  high performance liquid chromatography  Coreshell

摘要:

为同时测定柴辛注射液中有效组分甲基丁香酚、细辛脂素的含量，建立了以核壳型分离填料为色谱柱，乙腈和水为流动相，检测器波长为287 nm，流量为1.0 mL/min的高效液相色谱方法。结果显示，在0.15~150 μg/mL的质量浓度范围内，甲基丁香酚的线性曲线为Y=7 243X-258，r=0.9999，检测限为0.025 μg/mL，平均回收率在97.47%~100.53%之间，RSD为0.92%~2.17%；细辛脂素的线性曲线为Y=18 853X -676，r=0.9999，检测限为0.012 μg/mL，平均回收率在88.47%~92.23%之间，RSD为1.48%~3.55%。该方法操作简单，准确度、重复性、精密度好，可适用于柴辛注射液的质量控制。

Abstract:

In order to determine the methyleugenol and asarinin in Chaixin Injcetion, a high performance liquid chromatography(HPLC) method was established. Under the optimized conditions, the analyte was carried out with acetonitrile-water as a mobile phase at a flow rate of 1.0 mL/min and a wavelength of 287 nm on a coreshell column. The results showed good linear relationships of methyleugenol and asarinin within the mass concentrations of 0.15 to 150 μg/mL and the correlation coefficients were all more than 0.9999. The detection limits of methyleugenol and asarinin were 0.025 μg/mL and 0.012 μg/mL, while the average recovery of them were between 88.47% and 100.53%, with the relative standard deviations between 0.92% and 3.55%. The purposed method is simple to operate with good accuracy, repeatability, precision, which is suitable to the quality control.