鸡源大肠杆菌和沙门氏菌的分离鉴定及耐药分析

Study on the Antibacterial Effect of Compound Chinese Herbal Medicine on E.coli and Salmonella from Chicken

关键词:  肉仔鸡  大肠杆菌  沙门氏菌  分离鉴定

Key words:  Broiler  E. coli  salmonella  isolation and identification

摘要:

为探究甘肃省民勤县某饲养场肉仔鸡腹泻的病原及复方中草药制剂和抗生素分别对分离病原的抑菌效果,对7日龄病死肉鸡进行解剖，通过采集死亡肉仔鸡肝脏、脾脏、心脏进行病原菌分离培养、形态观察、16S rDNA分子鉴定，最终鉴定该病死肉仔鸡为大肠杆菌与沙门氏菌混合感染。抑菌结果显示，复方中草药对分离得到的大肠杆菌、鸡白痢沙门氏菌、鸡副伤寒沙门氏菌均有良好的抑菌效果。10种抗生素药敏试验结果显示，此三株菌同时对氨苄西林、环丙沙星、四环素、恩诺沙星四种药物耐药；鸡大肠杆菌对庆大霉素、阿米卡星敏感，鸡白痢沙门氏菌对链霉素、氟苯尼考、头孢噻肟敏感，鸡副伤寒沙门氏菌对头孢曲松、链霉素、头孢噻肟敏感。上述研究结果为肉仔鸡大肠杆菌与沙门氏菌混合感染的分离鉴定以及科学指导用药提供参考。

Abstract:

In order to explore the pathogenic effect of broiler chicken diarrhea and compound Chinese herbal preparations and antibiotics in a breeding farm in Minqin county, Gansu province, respectively, on the antibacterial effect of isolated pathogens, in this experiment, 7-day-old dead broilers were dissected. The liver, spleen and heart of dead broilers were collected for pathogenic bacteria isolation and culture, morphological observation, and 16S rDNA molecular identification. Finally, the dead broilers were identified as mixed infection of E. coli and Salmonella. The antibacterial results show that the compound Chinese herbal medicine has a good antibacterial effect on the isolated E. coli, Salmonella gallinarum and Salmonella paratyphi. The results of 10 antibiotic susceptibility tests showed that these three strains were simultaneously resistant to four drugs, ampicillin, ciprofloxacin, tetracycline, and enrofloxacin; Salmonella gallinarum is sensitive to streptomycin, florfenicol and cefotaxime, while Salmonella paratyphi is sensitive to ceftriaxone, streptomycin and cefotaxime. The above research results provide references for the isolation and identification of broiler chicken E. coli and Salmonella mixed infection and scientific guidance for medication.