河南省部分地区猪群大肠杆菌分离菌的耐药性比较分析

Comparative Analysis of Drug Resistance of Escherichia coli Isolated from Swine in Some Areas of Henan Province

关键词:  猪源大肠杆菌  耐药性  多重耐药

Key words:  swine Escherichia coli  drug resistance  multiple drug resistance

摘要:

为掌握河南省猪源大肠杆菌的耐药情况，于2013年至2018年对河南省郑州、开封、焦作、许昌四个地区部分规模化养猪场分离出的856株大肠杆菌，采用CLSI推荐的微量肉汤稀释法，调查其对8类13种代表性抗菌药物的耐药性。结果表明，856株大肠杆菌对氧氟沙星、黏菌素、庆大霉素、头孢噻呋和阿莫西林/棒酸等5种药物的耐药率在35.0%以下，分别为32.2%、31.1%、25.5%、15.5%和30.0%；对恩诺沙星、四环素、多西环素、大观霉素、氟苯尼考、磺胺异恶唑、复方新诺明和氨苄西林等8种药物的耐药率范围为45.0%~95.0%，其中对四环素、多西环素和磺胺异恶唑等3种药物的耐药率高达90.0%以上；多重耐药集中于耐5、6和7类抗生素，耐3类及以上药物的菌株占90.0%。本研究结果表明河南省部分养殖场猪源大肠杆菌耐药形势严峻，应加强对其监测和控制。

Abstract:

In order to understand the drug resistance of E.coli of pig origin in Henan Province, 856 strains of E.coli coli isolated from large-scale pig farms in Zhengzhou, Kaifeng, Jiaozuo and Xuchang, Henan province from 2013 to 2018 were investigated by using the microbroth dilution method recommended by CLSI to investigate the drug resistance of 13 representative antibiotics of 8 classes.The results showed that the resistance rates of 856 strains of E.coli to ofloxacin, colistin, gentamycin, ceftiofur and amoxicillin / clavulanic acid were less than 35.0%, which were 32.2%, 31.1%, 25.5%, 15.5% and 30.0% respectively, and to enrofloxacin, tetracycline, doxycycline,spectinomycin, florfenicol, sulfisoxazole, co-trimoxazole and ampicillin, etc The drug resistance rate ranged from 45.0% to 95.0%, among which the drug resistance rate to tetracycline, doxycycline and sulfisoxazole was more than 90.0%;the multiple drug resistance was concentrated on 5, 6 and 7 antibiotics, and 90.0% of the strains were resistant to 3 or more drugs. The results of this study showed that the situation of resistance of E. coli from pigs in some farms of Henan Province was severe, and the monitoring and control should be strengthened.