一种治疗奶牛阴道炎症微生态制剂的临床药效学研究

Clinical pharmacodynamic study of microecological preparation for treating vaginal inflammation in dairy cows

关键词:  微生态制剂  乳酸菌  临床  药效学

Key words:  Microecological preparation  Lactic acid bacteria  Clinical  Pharmacodynamics.

摘要:

摘要：为检验约氏乳杆菌SQ0048菌株所制备的微生态制剂对临床型奶牛阴道炎症的治疗作用，将其应用于患有阴道炎的病牛体内，利用临床兽医学方法观察给药前后患牛的临床症状；采用变性梯度凝胶电泳法检测给药前后患牛阴道内的微生物菌群种属；使用酶联免疫吸附技术检测给药前后患牛血清内细胞因子分泌量。结果显示，此微生态制剂不仅可以缓解临床奶牛阴道炎性症状，提高发情率及受胎率，还可以减少或杀灭阴道内的利氏卟啉单胞菌、铜绿假单胞菌、化脓隐秘杆菌及链球菌等，增加约氏乳杆菌SQ0048菌株及谷氨酸棒状杆菌的数量，改变阴道微生态环境，维持阴道的微生态平衡。同时给药后患牛血清中IL-1β、IL-6、IFN-α、TNF-α分泌量显著低于给药前，而IL-10分泌量则显著高于给药前，具有一定抑制炎症的生物学功能。试验表明，此微生态制剂可以缓解临床奶牛阴道炎性症状，改善阴道微生态环境，还具有一定的抑制炎症的生物学功能，可为其临床上的合理应用提供试验依据。

Abstract:

Summary:The aim of this study was to verify the therapeutic effect of microecological preparations prepared by using L. johnsonii SQ0048 strain on cow vaginal inflammation. The clinical symptoms of the cows vagina were observed by utilizing clinical veterinary methods denaturing gradient gel electrophoresis was used to detect microbial flora species in vaginal and enzyme-linked immunosorbent assays was used to detect the secretion of cytokines in bovine serum before and after Lactobacillus johnsonii SQ0048 was placed in the vaginal of diseased cow. The therapeutic effect of this microecological preparation was evaluated according to these changes before and after administration. The results show that the microecological preparation could not only relieve the clinical vaginal inflammatory symptoms in cows, improve the estrus and conception rate, but also kill or reduce the amount of main pathogenic bacteria Porphyromonas levii, Pseudomonas aeruginosa, Trueperella pyogenes and Streptobacillus sp, etc., increase the number of Lactobacillus johnsonii (SQ0048) and Corynebacterium glutamicum to improve vaginal micro-ecological environment and maintain the micro-ecological balance in the vaginal. At the same time, the secretion of IL-1β, IL-6, IFN-α and TNF-α was significantly lower than expected in bovine serum, while the secretion of IL-10 was significantly higher than expected. It had biological functions of inhibiting inflammation. The conclusion is the microecological preparation could alleviate the clinical vaginal inflammatory symptoms in cows, improve the vaginal micro-ecological environment, and also have certain biological functions of inhibiting inflammation. It might provide experimental basis for rational application in clinical practice.