基于中草药提取方法差异对鸡源变形杆菌的体外抑菌作用比较

Comparison of antibacterial effects in vitro of Chinese herbal medicine extraction methods on Proteus from chickens

关键词:  中草药  鸡源变形杆菌  抑菌作用  提取方法

Key words:  Chinese herbal medicine  Proteus vulgaris from chicken  Minimal inhibitory concentra-tion  Combined medicine sensitivity test.

摘要:

为探究中草药提取液对临床分离鸡源变形杆菌抑菌效果，本试验对野菊花、穿心莲等9种中草药，采取水提法和醇提法提取中草药的有效成分，并采用试管二倍稀释法测定中草药提取液对鸡源变形杆菌的最小抑菌浓度（MIC），采用微量肉汤稀释棋盘法对抑菌效果较佳的黄芩等5种水提液与虎杖等3种醇提液分别进行体外联合药敏试验，测定其FIC指数。结果表明：水提液中，黄芩抑菌效果最佳，MIC为62.5 mg/ml，穿心莲、虎杖、黄连、香薷次之，MIC均为125 mg/ml；醇提液中，虎杖、穿心莲、香薷抑菌效果较好，MIC均为125 mg/ml；体外联合药敏试验中，本试验各组合均无协同作用；说明黄芩水提液对鸡源变形杆菌抑菌效果最显著，其余抑菌效果一般或较差；醇提取液抑菌效果皆为一般或较差；本试验各联合用药效果皆不显著。

Abstract:

To study the antibacterial effect of Chinese herbal medicine extract on clinical isolates of Proteus, Nine kinds of Chinese herbal medicines were selected in this experiment, including Dendranthema indicum and Andrographis paniculate. The active constituents of Chinese herbal medicines were extracted by water extraction and alcohol extraction , and the minimum inhibitory concentration (MIC) of the herbal extracts against Proteus was determined by double dilution method. According to the results of MIC, choosing five water extracts including Scutellaria baicalensis Georgi etc, and three alcohol extracts, such as Reynoutria japonica Houtt. etc,which were combined in vitro for medicine sensitivity test, and their FIC index was measured. The results showed that the antibacterial effect of Scutellaria baicalensis Georgi was the best in the aqueous extract, the MIC was 62.5 mg/ml, and Andrographis paniculata, Reynoutria japonica Houtt, Coptis chinensis Franch and Elsholtzia ciliata (Thunb.)Hyland were next, MIC were 125 mg/ml. In the alcohol extract, Reynoutria japonica Houtt, Andrographis paniculata and Elsholtzia ciliata (Thunb.)Hyland had better bacteriostasis effect, MIC was 125 mg/ml. In vitro combined susceptibility test, there was no synergistic effect among the combinations in this experiment. This experiment showed that the water extract of Scutellaria baicalensis Georgi had the most significant bacteriostasis effect on Proteus from chickens, while the other medicine bacteriostasis effects were generally or poorly, the bacteriostatic effect of alcohol extract was generally or poorly, the combined effect of all medicine in this experiment was not significant.