悬浮MDCK细胞源与鸡胚源H9亚型禽流感疫苗免疫效力比较研究

Comparative Study on Immune Effiacay of MDCK Suspension Cell-derived and Chicken Embryo-derived H9 Subtype Avian Influenza Vaccine

关键词:  MDCK悬浮细胞  禽流感病毒  免疫剂量

Key words:  MDCK suspension cell  avian influenza virus  immune dose

摘要:

为探讨MDCK纯悬浮细胞制备的H9亚型禽流感灭活疫苗的免疫效果，使用10日龄SPF鸡胚和MDCK纯悬浮细胞分别接种H9亚型禽流感 BX13株病毒，收获抗原液，制备2种单价油佐剂灭活疫苗，按照不同免疫剂量对21日龄SPF鸡进行免疫接种，测定免疫后不同时间的血清HI抗体，免疫后21 d翅静脉攻毒，测定疫苗的保护率。结果表明：2种疫苗分别以0.3、0.2、0.1、0.02 mL/只的剂量接种21日龄SPF鸡后，7 d HI抗体均为0log2，免后14 d、21 d时2种疫苗相同免疫剂量组间HI抗体无明显规律性差异。免疫后21 d，2种疫苗0.1mL/只～0.3mL/只的免疫剂量，各组间HI抗体水平无明显差异，可以达到1∶128以上。攻毒试验表明，鸡胚源疫苗0.1 mL/只、MDCK细胞源疫苗0.2mL/只的免疫剂量可以达到10/10保护。本研究为使用MDCK纯悬浮细胞制备的H9亚型禽流感疫苗的深化研究和应用奠定了基础。

Abstract:

In order to evaluate the immune effectiveness of H9 subtype avian influenza inactivated vaccine prepared from pure suspension MDCK cells, the 10 days-old SPF chicken embryos and MDCK pure suspension cells were inoculated with H9 subtype AIV BX13 strain respectively. Two kinds antigen were harvested and with which two monovalent oil-based inactivated vaccine was prepared. 21 days-old SPF chickens were immunized with the two vaccine and with different immunizing doses. HI titers were tested at 7 days period after immunization, and protection rate were tested by challenge the chickens with the same AIV strain to the vaccine antigen by intravenous route at 21 days after immunization. The results showed there is no HI titers were detected 7 days after immunization of the two vaccines with the 4 inoculation doses. There was no significant HI titer difference between the two vaccines when immunized with the same doses 14 days and 21 days after inoculation. When immunized the two vaccine with 0.1 mL/chicken～0.3 mL/chicken both vaccine can stimulate the chickens produce more than 1∶128 HI titer. The challenge results indicated that 10/10 protection were obtained for the chicken embryo-derived vaccine when immunized with more than 0.1 mL/chicken and more than 0.2 mL/chicken for the cell-derived vaccine. This study laid a foundation for further research and application of H9 subtype avian influenza vaccine prepared by MDCK pure suspension cells.