一种猪瘟兔化弱毒活疫苗效力检验方法的研究

Study on a method for detecting the titer of live attenuated swine fever vaccine

Key words:  IFA, Classical  swine fever  vaccine, Titer  test

摘要:

现有猪瘟活疫苗的效力检验多采用兔体定型热反应法(RID),但该方法存在重复性差、检验周期长、数据不精确等缺点,为克服兔体定型热反应法存在的缺陷,本研究建立了一种快速、准确、稳定的检测猪瘟病毒含量及疫苗效力的间接免疫荧光方法(IFA),该方法以出现特异性荧光作为检测标准,通过添加促细胞感染剂PB增加特异性荧光数量,可以在4d内检测出猪瘟活疫苗成品或半成品病毒含量,该方法检验周期短、数据精确、重复性好,为猪瘟活疫苗效力检验方法的完善提供了有力支持。

Abstract:

In order to overcome the defects of the existing methods for testing the effectiveness of classical swine fever vaccine, a rapid and stable method for detecting the titer of classical swine fever vaccine was established. In this method, specific fluorescence was used as the standard of detection, and the specific fluorescent quantity was increased by adding PB, and titer of swine fever live vaccine could be tested in 4 days. The method established in this study can meet the needs of batch detection, and provide a reference for the further improvement of the detection method.