不同生长年限黄檗树枝与关黄柏中生物碱类成分相关性研究

Study on the relationship between the alkaloids in Phellodendron mandshurica and the branches of Phellodendron amurense Rupr. in different growth years

关键词:  黄檗  关黄柏  生物碱类  不同年限  指纹图谱

Key words:  Phellodendron amurense Rupr.  cortexr phellodendri  alkaloids  different years  fingerprint

摘要:

建立黄檗树枝与关黄柏中5种生物碱类成分的含量测定方法,比较关黄柏、黄檗三年生、四年生、七年生及八年生树枝中生物碱类成分含量累积规律。采用HPLC法对不同年限的黄檗树枝与关黄柏中5种生物碱类成分进行含量测定；采用相似度比较法进行分析及共有峰的鉴定；采用质谱法对未标定成分进行分析。随着生长年限增长,黄檗树枝中盐酸黄柏碱、木兰花碱、盐酸药根碱、盐酸巴马汀、盐酸小檗碱的含量逐年递增。八年树枝与关黄柏药材共有模式之间的相似度大于0.96。建立的不同年限黄檗树枝与关黄柏中5种生物碱类成分的含量测定方法简便、准确、重复性好。黄檗树枝与关黄柏主要成分较为相近,且八年生生物碱含量与关黄柏药材最为相近,可进一步为关黄柏药源高值化利用提供理论依据。

Abstract:

To establish a method to determine the content of 5 alkaloids in the branches of Phellodendron amurense Rupr. and cortexr phellodendri,and to compare the accumulation of alkaloids in three years old, four years old, seven years old and eight year old tree branches of between Phellodendron amurense Rupr.and cortexr phellodendri. HPLC method was used to determine the content of 5 alkaloids in berberis and cypress in different years. The similarity comparison method was used to analyze and identify the common peaks. The uncalibrated components were analyzed by mass spectrometry. With the growth of years, the content of amylopecine hydrochloride, magnolidenine, rhizine hydrochloride, bamatine hydrochloride and berberine hydrochloride in the branches of berberine is increasing year by year. The similarity between the common pattern of 8 years branch and cypress was greater than 0.96.The method to determine the content of 5 alkaloids in berberis and cypress in different years is simple, accurate and reproducible. The main components of berberine and cypress cypress were similar, and the content of 8-year alkaloid was similar to that of cypress cypress.