茶树油提取物粉对小鼠免疫功能的影响

Effect of the Powder of Tea Tree Oil Extract on the Immune Function of Mice

关键词:  茶树油提取物  小鼠  免疫功能  淋巴细胞增殖  血清溶血素  巨噬细胞吞噬

Key words:  Tea tree oil extract powder  Mice  Immune function  Lymphocyte proliferation  Serum hemolysin  Macrophage phagocytosis

摘要:

研究茶树油提取物粉对小鼠免疫功能的影响，为茶树油产品的研发及作为畜禽养殖添加剂的应用积累资料。茶树油提取物粉对小鼠的经口急性毒性LD50﹥5000 mg/kg b.w.。茶树油提取物粉分别以1250 mg/kg b.w.、250 mg/kg b.w.、50 mg/kg b.w.灌胃给予连续10天，分别进行腹腔巨噬细胞吞噬活性和脾淋巴细胞增殖试验，测定免疫器官指数、血清溶血素水平及外周血CD3+、CD4+、CD8+T淋巴细胞亚群水平。结果表明茶树油提取物粉高、中、低剂量组能极显著增加小鼠脾脏指数（P<0.01），中剂量能显著增加肝脏指数和胸腺指数（P<0.05）。中、高剂量能显著提高腹腔巨噬细胞吞噬活性和血清溶血素水平（P<0.05）。三个剂量组均能显著提高小鼠脾淋巴细胞增殖的刺激指数（P<0.05）。中剂量组能显著增加小鼠CD4+/CD8+比值（P<0.05）。结论是茶树油提取物粉在一定程度上能够提高小鼠细胞免疫和体液免疫，具有促进小鼠免疫功能的作用。

Abstract:

To study the effect of the tea tree oil extract powder on the immune function of mice, and to accumulate data for the research and development of the tea tree oil products and the application as additives for livestock and poultry breeding. Acute oral toxicity of the tea tree oil extract powder to mice is LD50>5000 mg/kg b.w. ICR mice were orally administered at dose of 1250, 250 and 50 mg/kg b.w. for 10 consecutive days respectively, the peritoneal macrophage phagocytosis activity and spleen lymphocyte proliferation test were performed respectively, and the immune organ index, serum hemolysin levels and level of CD3+, CD4+, CD8+ T lymphocyte subsets in peripheral blood were determined. The results showed that the high, medium and low doses of the tea tree oil extract could significantly increase the spleen index of mice (P<0.01), and the medium dose could significantly increase the liver index and thymus index (P<0.05). Medium and high doses of the tea tree oil extract could significantly increased the peritoneal macrophage phagocytic activity and serum hemolysin level(P<0.05).The stimulation index of mouse spleen lymphocyte proliferation was significantly increased in all three dose groups (P<0.05).The middle dose group significantly increased the CD4+/CD8+ ratio in mice (P<0.05).The conclusion is that the tea tree oil extract powder can improve the cellular immunity and humoral immunity of mice to a certain extent, and has the effect of promoting the immune function of mice.