高效液相色谱法测定6种兽用中药散剂中的利福平

Establishment of Detection Methods in the six Veterinary Traditional Chinese Herb Medicine Powders by HPLC

关键词:  高效液相色谱仪  兽用中药制剂  利福平

Key words:  rifampicin  HPLC  veterinary traditional chinese medicine preparation

摘要:

为建立麻黄鱼腥草散、鸡痢灵散、定喘散、银翘散、金花平喘散、四黄止痢颗粒六种中兽药散剂中非法添加利福平高效液相色谱检查方法,研究使用C₁₈(250 mm × 4.6 mm,5μm,)色谱柱,样品经前处理后用流动相稀释［乙腈：甲醇：磷酸盐缓冲液=40:40:20］,采用二极管阵列检测器,波长采集范围为 200 ～ 400 nm,记录色谱图波长为 237 nm,流速为 1 mL/min,进样量为10μL。结果表明,利福平进样浓度在5～226μg/ml范围内与峰面积呈良好的线性关系(r₂=0.9999),平均回收率为93.4%(n= 6),RSD为0.6%。本方法简便、准确、可行,适用于兽用中药制剂中非法添加利福平的定性定量检测。

Abstract:

A detection method of rifampicin illegally adulterated in the six veterinary traditional Chinese herb medicine. It was established by HPLC.The six veterinary traditional Chinese Herb medicine in cluding Manghuangyuxingcao San,Jililing San, Dingchuan San,Yinqiao San,Jinhuapingchuan San, Sihuangzhilikeli. Samples directly diluted by mobile phase (acetonitrile: methanol :phosphatebuffer=40:40:20),high performance liquid chromatography with diode array detector. The results showed rifampicin in 0.5 ～260μg/m L concentration range of linear relationship is good; 6 kinds of veterinary traditional chinese herb medicine preparation recoveries in 93.4%,the coefficient of variation in 0.6%.The method has good recovery rate, accuracy is higher, which can be used for qualitative and quantitative detection of rifampicin illegally added in veterinary traditional chinese medicinal preparation.