木犀草素联合庆大霉素对耐药化脓隐秘杆菌抑制作用的研究

Study on Inhibiting Effects of Luteolin Combined with Gentamicin Against Drug-resistant Trueperella pyogenes

关键词:  木犀草素  化脓隐秘杆菌  药物联合  抗菌作用  逆转耐药

Key words:  luteolin  T. pyogenes  combination between antimicrobial agents  antibacterial effect  reversing drug resistance

摘要:

为探究木犀草素联合庆大霉素对耐药化脓隐秘杆菌的抑制作用，采用微量肉汤稀释法分别测定木犀草素和庆大霉素对34株化脓隐秘杆菌的最小抑菌浓度（MIC）。选取对庆大霉素耐药的化脓隐秘杆菌，通过棋盘法测定两种药物联合抑菌效果。经亚抑菌浓度木犀草素作用耐药菌株，比较木犀草素作用前后化脓隐秘杆菌对庆大霉素的耐药性变化情况。结果表明，木犀草素对化脓隐秘杆菌的MIC值为39～156 μg/mL，与庆大霉素联合的作用为不相关；耐药菌经亚抑菌浓度木犀草素（1/4MIC）作用36 h后，庆大霉素对耐药菌的MIC值下降2～32倍，且原菌株耐药水平越高，MIC下降倍数越高。提示木犀草素具有良好抗菌活性，且能够逆转化脓隐秘杆菌耐药性。

Abstract:

To explore the antibacterial effects of luteolin combined with gentamicin，the minimum inhibitory concentrations (MICs) of luteolin and gentamicin against 34 strains of Trueperella pyogenes(T. pyogenes) were tested by broth microdilution method. The gentamicin resistance isolates were selected to determine the co-antibacterial effects between luteolin and gentamicin by the chessboard method. The drug-resistant levels among these isolates were observed after treating with luteolin at a sub-inhibitory concentration. The results indicated that the MIC of luteolin ranged from 39 to 156 μg/mL, meanwhile there was no co-effects against T. pyogenes between luteolin and gentamicin. The MICs of these resistant isolates become 1/2 to 1/32 after treating 36 h with a sub-inhibitory concentration (1/4MIC) of luteolin, the MICs decreased significantly as the antibiotic resistant levels become higher. The results show that luteolin has a good antibacterial activity and it can reverse the drug resistance of T. pyogenes.