黄秋葵醇提物对顺铂致急性肾损伤的保护作用

Protective effects of alcohol extract of okra cisplatin induced acute kidney injury

关键词:  顺铂  黄秋葵醇提物  肾毒性  血尿素氮  肌酐

Key words:  cisplatin  okra extract  renal toxicity  blood urea nitrogen  serum creatinine

摘要:

探讨黄秋葵(Abelmoschus esculentus Linn．)嫩果醇提物对顺铂致小鼠急性肾损伤是否有保护作用。将50只SPF级ICR雄性小鼠随机均分为空白对照组（0.9%生理盐水）、模型组（0.9%生理盐水）、黄秋葵醇提物（HQK）高中低剂量组（400、200、100 mg?kg-1）,分别灌胃给药7天，除空白对照组，于第7天给药1 h后灌胃给顺铂20 mg?kg-1。再灌胃给药3天后，眼球取血，测定血清中尿素氮（BUN）、肌酐（CRE）、超氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）和丙二醛（MDA）的含量；测定肾脏匀浆中SOD、GSH和MDA含量；并观察苏木精-伊红(HE)病理切片组织病理学变化。本实验结果表明：与模型组比较，HQK高、中剂量组中BUN、CRE含量均降低，肾组织GSH、SOD含量均升高（P＜0.01，P＜0.05)，HE染色观察HQK高剂量组可显著保护顺铂致肾组织病理损害。黄秋葵醇提取物对CDDP诱导的小鼠急性肾损伤均有一定的保护作用。

Abstract:

The study of okra tender fruit alcohol extract protective effects against cisplatin-induced acute kidney injury in mice．The 50 SPF ICR male mice were randomly divided into five groups:control (0.9% normal saline), model (0.9% normal saline), okra alcohol extraction from high, medium and low dose group（400、200、100 mg?kg-1）. Respectively, 7 days of intragastric administration. 7 days, in addition to the control group, were given one hour after administration of gastric administration of cisplatin 20 mg?kg-1. And then intragastric administration 3 days，after eyeball plasma, serum centrifugal stay. We tested the content of serum blood urea nitrogen(BUN) ,serum creatinine (CRE),superoxide dismutase(SOD),glutathione (GSH) and malondialdehyde(MDA).Also measured SOD, GSH and MDA content in kidney homogenates; and inspected the changes of pathological by hematoxylin-eosin (HE). The experimental results show ,compared with the model, the okra alcohol extract high and middle dosage group, BUN, CRE content decreased and kidney tissues by GSH, SOD levels were increased(P＜0.01，P＜0.05). We discovered the okra alcohol extract better than the model could protectd renal pathological damage by HE . Therefore Okra alcohol extract on cisplatin-induced acute kidney injury in mice has a protective effect.