| 摘要: |
| 为制备鸭瘟种毒和鸭瘟活疫苗检验用抗鸭瘟病毒特异性阳性血清，对6个月龄左右的山羊进行五次免疫。最后一次免疫后2周采血并分离血清，血清经混合、分装、冷冻真空干燥后，对其进行了无菌检验、外源病毒检验、剩余水分测定、中和效价测定、均匀性检验。结果表明，本研究制备的抗鸭瘟病毒阳性血清特异性良好，无菌检验、外源病毒检验和剩余水分测定均符合《中华人民共和国兽药典》（2010年版三部）规定；血清中和效价大于1∶100，均匀性良好，可满足兽药典标准中鸭瘟种毒和鸭瘟活疫苗的鉴别检验和外源病毒检验。 |
| 关键词: 鸭瘟 特异性血清 检验 |
| DOI： |
| 投稿时间：2015-12-31修订日期：2016-03-21 |
| 基金项目:“十二五”农村领域国家科技计划课题（2015BAD12B03-07），948项目（2011-G14（4）） |
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| Preparation and Identification of Specific Antiserum against Duck Plague Virus for Test |
|
| (China Institute of Veterinary Drug Control) |
| Abstract: |
| To prepare the specific antiserum against duck plague virus (DPV) for test, goats with about 6 months old were inoculated with live and inactivated DPV. 2 weeks after the last immunity, serums were collected and mixed thoroughly. After lyophilization, the serum was used for sterility test, exogenous virus test, residual moisture test, neutralization titer determination and uniformity test. The results of sterility test, exogenous virus test, residual moisture testcomplied with China veterinary pharmacopoeia. The neutralization titer was higher than 1∶100. The antiserum against DPV was prepared with high specificity and good uniformity for the identifying inspection and exogenous virus test of duck plague live vaccines and virus seeds. |
| Key words: duck plague virus specific antiserum test |
