磺胺嘧啶钠与兔血浆蛋白结合率的检测研究

Determination of the Binding Rate of Rabbit Plasma Protein with Sulfadiazine Sodium

关键词:  磺胺嘧啶钠  血浆蛋白结合率  平衡透析法，兔

Key words:  sulfadiazine sodium(SD)  protein binding rate  equilibrium dialysis  rabbit

摘要:

为研究磺胺嘧啶钠与兔血浆中血浆蛋白结合情况，采用平衡透析法和超滤法模拟磺胺嘧啶钠与血浆蛋白结合过程，高效液相色谱法测定透析内液及外液中药物浓度，计算磺胺嘧啶钠血浆蛋白结合率。结果表明：血浆中其他成分对测定无干扰，各待测物在选择浓度范围内线性关系均良好，精密度及稳定性结果均符合方法学要求。透析外液线性范围为0.5～20 μg/mL，透析内液的线性范围为1～100 μg/mL，提取回收率在78.67%～98.36%之间，日内、日间变异均小于10%，磺胺嘧啶钠的体外血浆蛋白结合率平均为32.47%，体内血浆蛋自结合率平均为36.43%。本试验建立的方法简单，灵敏、专属性强，磺胺嘧啶钠在兔血浆中蛋白结合率较低，且具有浓度依赖性。

Abstract:

To study the plasma protein binding rate of sulfadiazine sodium in rabbit plasma. The equilibrium dialysis method and the ultrafiltration method were used to imitate the binding process between sulfadiazine sodium and plasma protein, The HPLC was adopted to determined sulfadiazine sodium in plasma. The results showed that there was no interference from other ingredients for the determination of sulfadiazine sodium. The calibrationcurve of the analytes was in good linearity in certain range of contents. The precision and stability of the analytes met the methodology requirements. The calibration curve of the buffer solution was linear in the range of0.5～20 μg/mL,The calibration curve of the plasma was linear in the range of 1～100 μg/mL,The extract recovery was 78.67%～98.36%,RSDs of infra and inter-day precisions were all less than 10%.The binding rates of plasma protein with sulfadiazine sodium in vitro was 32.47% and in vivo was 36.43%. The HPLC method established in the paper is simple sensitive and stable. SD has a lower protein binding rate with rabbit plasma and The binding of SD to plasma protein was concentration dependent.