猪肺炎支原体DJ-166株的分离鉴定

Isolation and Identification of Mycoplasma hyopneumoniae DJ-166 Strain

关键词:  猪肺炎支原体  分离鉴定  

Key words:  Mycoplasma hyopneumoniae  Isolation and Identification  

摘要:

从山西一猪场采集疑似猪支原体肺炎肺组织病料,接种CH培养基培养,经过克隆纯化获得一株菌株；该菌株经PCR、多重PCR、培养特性、生化特性和血清学特性试验鉴定为猪肺炎支原体,命名DJ-166株。该分离菌株在人工合成培养基上传8代稳定后,活菌计数达到108CCU/ml；菌液培养物气管注射猪后,致病性较弱；免疫原性试验证明该分离菌株具有良好的免疫原性,为猪支原体肺炎疫苗的研究提供了必要条件。

Abstract:

A suspected pig pneumonia pathological tissue was collected from a swine farm of Shanxi province, from which a strain was cloned and purified by CH medium culture. The strain was further identified to be Mycoplasma hyopneumoniae by PCR, multiple PCR, biochemical character and serological specificity experiment, then named after DJ-166 strain. After 8 generation stable culture on artificial synthetic medium, viable count reached 108 CCU/ml. The culture showed weak pathogenicity when being injected into the swine by weasand. Immunogenicity test showed that the strains has good immunogenicity, and provided the necessary condition for Mycoplasmal pneumonia vaccine research.