一株新型鸭肝炎病毒的分离与鉴定

Isolation and Identification of a Duck Hepatitis Virus Strain

关键词:  鸭肝炎病毒  分离  鉴定

Key words:  duck hepatitis virus  Isolation  Identification

摘要:

从辽宁省某鸭场疑似鸭病毒性肝炎病料中分离到一株病毒，经PCR检测初步鉴定其为鸭肝炎病毒，命名为YK株，应用易感鸭胚和雏鸭进行传代及病毒含量测定，E1～E5代鸭胚适应毒病毒含量在106.5～106.9 ELD50/0.2 mL之间，E2代鸭肝组织毒病毒含量为106.3 LD50/0.1 mL。动物试验结果表明，YK株鸭胚适应毒E2代对7日龄雏鸭的致死率高达100%。序列测定分析表明，YK株与DHV-A的VP1基因同源性在71.4%～72.3%之间，与DHV-B的VP1基因同源性在71.9%～72.3%之间，与DHV-C的VP1基因同源性在94.3～98.8%之间。实验表明，YK株与韩国新型鸭肝炎病毒在同一分支上，属于基因C型DHV。

Abstract:

A virus strain was isolated from suspected duck viral disease from dead duck in liaoning Province,and identified by reverse-transcriptase polymerase chain reaction.It is a duck hepatitis virus that named YK strain.The isolates were passed from E1 to E5,the titer determination in duck embryos were from 106.5 to 106.9 ELD50/0.2 mL; the titer determination in duckling hepatitis tissue titer was 106.3/0.1mL at E2. Animal regression experimental results showed that The YK strain in duck embryos to 7- day- old duckling mortality was 100%.Sequence comparison with DHV-A serotype showed that the identity was 71.4%～72.3% , 71.9%～72.3% with DHV-B serotype and 94.3～98.8% with DHV-C serotype.It proves that the YK strain and new serotype strain in Korea belongs to DHV-C serotype.