双葛止泻口服液抑菌剂用量筛选与抑菌效力研究

Study on dosage screening of antimicrobial preservatives and antimicrobial effectiveness of Shuangge Zhixie Oral Liquid

关键词:  双葛止泻口服液  剂量筛选  抑菌效力  苯甲酸钠

摘要:

通过抑菌效力研究确定了双葛止泻口服液中抑菌剂的合理添加量。根据《中国兽药典》2015年版一部附录“1121抑菌效力检查法”要求,以金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌、白色念珠菌和黑曲霉为试验菌株,建立双葛止泻口服液抑菌效力检查方法,对不同苯甲酸钠含量的双葛止泻口服液进行抑菌效力测定,筛选出供试品中抑菌剂合理添加量。结果表明,0.3%的苯甲酸钠对微生物生长繁殖具有良好的抑制作用,且为最低有效剂量。因此0.3%的苯甲酸钠可作为双葛止泻口服液的抑菌剂用量。

Abstract:

The reasonable addition amount of the antimicrobial preservative added to Shuangge Zhixie Oral Liquid was determined by studying the antimicrobial effectiveness. According to the appendix 1121(method for antibacterial efficacy test) of Chinese Veterinary Pharmacopoeir(2015 edition,volume Ⅰ),Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans and Aspergillus niger were used as test strains to establish a method for checking the antibacterial