一例鸡源致病型大肠杆菌的分离鉴定

Isolation,Identification and Drug Sensitivity Test of Escherichia coli from Hens

Key words:  Hens  E. coli  Isolation and identification  Drug sensitivity test

摘要:

为进行一例蛋鸡病原菌感染情况确定及病原菌的敏感药物筛选,无菌采集疑似大肠杆菌蛋鸡病例的肝、脾等组织,进行病原菌的分离培养,经过培养特性检查、菌落形态观察、革兰氏染色鉴定、生化特性考察、PCR鉴定、测序分析以及动物致病性试验,确定分离菌株为大肠杆菌。选用10种临床常用抗菌药物进行药敏试验,药敏试验结果表明,分离菌株对青霉素、氟苯尼考、阿莫西林等多种抗生素产生耐药性,对丁胺卡那敏感。

Abstract:

In order to identify the pathogenic bacteria and screen of sensitive drugs for pathogenic bacteria, the liver and spleen of suspected E. coli hens were aseptically collected, and the pathogens were isolated and cultured. After culture characteristics examination, colony morphology observation, Gram staining identification, biochemical property investigation, PCR identification, sequencing analysis and animal pathogenicity test confirmed that the isolated strain was Escherichia coli. Ten clinically used antibacterial drugs were selected for drug sensitive test. The results of drug sensitive test showed that the isolated strains were resistant to various antibiotics such as penicillin, florfenicol and amoxicillin, and sensitive to amikacin.