翼草景香水煎液对多杀性巴氏杆菌体外抑菌活性及小鼠感染模型的防治效果研究

Study on the Antibacterial Activity of Yicaojingxiang Decoction against Pasteurella multocida in Vitro and the Prevention and Treatment Effect of Mouse Infection Model

关键词:  翼草景香  多杀性巴氏杆菌  最小抑菌浓度  感染模型  抑菌活性

Key words:  Yicaojingxiang  Pasteurella multocida  Minimal inhibitory concentration  Infection Model  Antibacterial activity

摘要:

研究翼草景香水煎液对多杀性巴氏杆菌的体外抑菌活性及小鼠感染模型的防治效果。利用传统水煎法制备翼草景香水煎液，用HPLC方法测定翼草景香水煎液中红景天苷浓度，并用红景天苷浓度标定翼草景香水煎液药物浓度，分别采用牛津杯法和试管2倍稀释法测定翼草景香水煎液对多杀性巴氏杆菌抑菌圈直径和最小抑菌浓度（MIC），通过建立死亡率为75%的多杀性巴氏杆菌体内感染小鼠模型，测定翼草景香水煎液浓度为0.12316 mg/mL时，在感染前和感染后给药方式下，分别给与0.1 mL、0.3 mL、0.5 mL药物时对小鼠的体内感染防治作用。结果表明，当翼草景香水煎液浓度为0.49263 mg/mL时，抑菌圈平均直径为28.5 mm；MIC值为0.00385 mg/mL，经病理解剖、细菌镜检、全自动微生物鉴定及药敏系统（VTEK 2 COMPACT）系统细菌鉴定，表明死亡率为75%的多杀性巴氏杆菌体内感染小鼠模型建立成功，当水煎液浓度为0.12316 mg/mL时，0.1 mL、0.3 mL、0.5 mL预防组和治疗组小鼠死亡率分别依次为50%，41.7%，81.8%，66.7%，50%和75%。因此，翼草景香水煎液对多杀性巴氏杆菌具有良好的体内、外抑菌活性，且其预防作用较治疗作用明显。

Abstract:

To clarify the antibacterial activity of the Yicaojingxiang decoction on Pasteurella multocida in vitro and prevention and treatment of infection in vivo. Extracting the effective components with water-extracting in the compound, the content of Salidroside in Yicaojingxiang decoction were measured by HPLC method, and its content indicates the drug concentration of Yicaojingxiang decoction. The antibacterial ring diameters and minimal inhibitory concentration (MIC) were determined by Oxford plate assay system and tube dilution; Establishing the model that lethality rate is 75% of Pasteurella multocida infection in mice and measuring the prevention and treatment of infection in vivo in different doses of the experimental group when the decoction concentration is 0.12316 mg/mL. When Yicaojingxiang decoction concentration is 0.49263 mg/mL, the average diameter of antibacterial rings was 28.5mm, and the MIC was 0.00385 mg/mL. Pathological examination, bacterial microscopy, VITEK 2 COMPACT identification after infection showed that the mice infected with Pasteurella multocida model was successfully established. When the concentration of decoction was 0.12316 mg/mL, the mortality of the 0.1 mL, 0.3 mL, 0.5 mL prevention group and the treatment group were 50%, 41.7%, 81.8%, 66.7%, 50% and 75%, respectively. Comprehensive test results，Yicaojingxiang decoction have a good antibacterial activity on Pasteurella multocida in vitro and in vivo, and its preventive effect is more obvious than the therapeutic effect.