板蓝根超微粉粉体特性及水溶性成分溶出研究

Study on Characteristics and Water-soluble Components of Ultrafine Radix isatidis

关键词:  板蓝根  超微粉碎  粉体特性  活性成分

Key words:  Radix isatidis  ultrafine grinding  powder characterization  bioactivity

摘要:

通过对板蓝根超微粉碎粉体特性研究，为中药超微粉推广应用提供依据。试验通过扫描电镜、激光粒度分析仪及高效液相色谱对板蓝根超微粉、板蓝根普通粉的粒度及水溶性活性成分溶出进行检测,探讨超微粉碎对中药粉碎粒度、活性成分溶出影响。试验结果显示板蓝根超微粉和普通粉D50分别为5.6 μm、171.058 μm；比表面积分别为1.551 cm2/g、0.225 cm2/g；休止角分别为47.43°、42.95°；松密度分别为0.39 g/cm3、0.48 g/cm3。电镜观察超微粉看不到完整细胞结构，多为细胞碎片，而普通粉可以看到完整的细胞。板蓝根超微粉和普通粉水溶性浸出物的溶出量大约为50%和42%。板蓝根超微粉和普通粉多糖提取率分别为9.34%和8.36%。板蓝根超微粉和普通粉中(R,S)-告依春的含量1.068 mg/g、0.784 mg/g。结论：板蓝根经过超微粉碎后，中直粒径可达5.6μm，细胞破壁率可达100%，而普通粉破壁率仅为31.74%；板蓝根经超微粉碎对多糖的溶出显示为超微粉大于普通粉，板蓝根超微粉水溶性活性成分告依春溶出率优于普通粉。

Abstract:

The purpose of this experiment is to detect the particle size and water-soluble active components of ultrafine Radix isatidis and ordinary Radix isatidis by using the scanning electron microscopy, laser particle size analyzer and high performance liquid chromatography. The results showed that the D50 of ultrafine Radix isatidis and ordinary Radix isatidis were 5.6 μm and 171.058 μm respectively. The specific surface areas were 1.551 cm2/g and 0.225 cm2/g respectively. The angle of reposes were 47.43°、42.95° respectively. The bulk density were 0.39 g/cm3 and 0.48 g/cm3 respectively. The complete cell structure of ultrafine Radix isatidis could not be observed and mostly were cell debris through the electron microscopy observation of the ultrafine Radix isatidis, while the complete cell structure could be observed through the electron microscopy observation of the ordinary Radix isatidis.The dissolution rate of the ultrafine Radix isatidis and ordinary Radix isatidis water-soluble extract were about 50% and 42%. The extraction rates of ultrafine and ordinary powder polysaccharides were 9.34% and 8.36% respectively. The content of (R, S)-epigoitrin in ultrafine Radix isatidis and ordinary Radix isatidis were 1.068 mg/g and 0.784 mg/g. Conclusion: After the ultra-fine pulverization of Radix Isatidis, its medium diameter is up to 5.6μm, the cell wall-broken rate is up to 100%, while the cell wall-broken rate of ordinary Radix isatidis is only 31.74%, and for the dissolution of the polysaccharide, ultrafine Radix isatidis is more than the ordinary Radix isatidis. For the dissolution rate of water soluble active ingredient epigoitrin of Radix Isatidis, ultrafine powder is better than that of ordinary powder.