| 摘要: |
| 建立了兽药中非法添加药物阿托品的超高效液相色谱-串联质谱（UPLC-MS/MS）的检测方法。样品经提取稀释，采用BEH C18柱为分离柱，外标法定量。结果表明，阿托品在0.5～10 ng/mL范围内线性关系良好，相关系数大于0.99。在4～6 mg/g(或mg/mL)添加浓度范围内阿托品的平均回收率97.4%～110.9%，批内变异系数均小于10％，检测限为0.2 mg/g(或mg/mL)，定量限为0.5 mg/g(或mg/mL)。该方法简便、灵敏度高、重现性好，适用于兽药中非法添加阿托品的含量测定。 |
| 关键词: 阿托品 兽药 超高效液相色谱-串联质谱 |
| DOI： |
| 投稿时间：2014-07-27修订日期：2014-10-15 |
| 基金项目:2014年国家畜禽产品质量安全风险评估项目（GJFP2014007） |
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| Determination of Atropine Illegally Added in Veterinary Drugs by UPLC-MS/MS |
|
| (Henan Institute of Veterinary Drug Control) |
| Abstract: |
| A method based on ultra performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) was developed for determination of atropine in veterinary drugs. The analyte was extracted and diluted with water，with BEH C18 as the analytical column. Quantification of the analyte was using external standard method. The results indicated that the correlations of atropine were greater than 0.99 in the range of 0.5～10 ng/mL. The average recoveries of the drug ranged from 97.4%～110.9% at spiked levels of 4～6 mg/g(mg/mL) with RSD less than 10%. The detection limit and quantification limit of the method were 0.2 mg/g(mg/mL) and 0.5 mg/g(mg/mL). The method was convenient，rapid，sensitive and reproducible for monitoring atropine illegally added in veterinary drugs. |
| Key words: atropine veterinary drugs UPLC-MS/MS |
