五种兽药中非法添加喹乙醇和乙酰甲喹的HPLC-PDA检测方法的建立

Determination of Olaquindox and Mequindox Illegally AddedSin Five Kinds of Veterinary Drug by HPLC-PDA

关键词:  喹乙醇  乙酰甲喹  高效液相色谱法  二极管阵列检测器  峰纯度检查  光谱相似度检查

Key words:  olaquindox  mequindox  HPLC  photo-diode array detector  peak purity test  spectrum similar test

摘要:

建立了高效液相色谱法测定五种制剂中非法添加喹乙醇与乙酰甲喹的方法。以十八烷基键合硅胶为填充剂,乙腈-0.01mol/L磷酸二氢钾(pH 6.0)为流动相,二极管阵列检测器进行全波长(200～400nm)扫描,检测波长为260nm,并采用峰纯度检查和光谱相似度检查辅助对照品比对方法,对非法添加药物进行确证。结果表明,该色谱条件下喹乙醇、乙酰甲喹与其他物质峰分离良好。喹乙醇、乙酰甲喹在0.5～200μg/mL浓度范围内线性良好,回收率在91.1%～105.7%之间,RSD≤3.1%,喹乙醇、乙酰甲喹的检测限分别为1.0mg/g、2.5 mg/g。

Abstract:

ASmethod of illegally adding olaquindox and mequindoxSin the five kinds of preparationsSwhich using the high performance liquid chromatography was studied.SIt was tested with C18 column,S using isocratic elution with 0.01mol/L potassium dihydrogen phosphate (pH6.0) and acetonitrile as the mobile phase, diode array detector scanning 200～400 nm, the detection wavelength was 260nm.SPeak purity test and spectrum similar test were helped to identify the olaquindox and mequindox. The results showed that under the chromatographicScondition,Solaquindox、 mequindox and other materials separated well.SThe linearitySof olaquindox、mequindox in theSrangeSof 0.5～200.0 μg/mL were good, the mean recovery of olaquindox、 mequindox were 91.1%～S105.7%,RSD≤3.1%,the detection limits of olaquindox and mequindox were 1.0 mg/g、2.5 mg/g.