卡洛芬注射液在奶牛体内的残留消除研究

Study on Residual Elimination of Carprofen Injection in Dairy Cows

关键词:  卡洛芬  奶牛  残留消除  休药期

Key words:  carprofen  dairy cows  residual elimination  withdrawal period

摘要:

本研究旨在分析卡洛芬注射液在奶牛体内的残留消除规律并确定休药期。24头健康奶牛按1.4 mg/kg体重颈部皮下注射卡洛芬，单次给药后分别在12 h、3 d、7 d、14 d、21 d时间点采集奶牛的肌肉、肝脏、脂肪和肾脏样品。组织样品经处理后，采用建立的超高效液相色谱串联质谱法(UPLC-MS/MS)检测卡洛芬的含量。方法学结果表明：在200～2000 ng/mL的添加范围内，方法的线性关系良好，相关系数r≥0.99。不同组织中卡洛芬的平均回收率在87.79%~113.83%之间，批内批间变异系数均小于15%。经休药期计算软件WT1.4计算，肌肉、肝脏、肾脏、脂肪中的休药期分别为7.75、10.04、10.25和7.54 d。21 d时注射部位的残留量低于肌肉中的最大残留限量。因此，按推荐给药方案在奶牛中使用卡洛芬注射液，建议将休药期定为21 d。

Abstract:

The purpose of this study is to analyze the residual elimination rule of carprofen injection in dairy cows and determine the withdrawal period. 24 healthy dairy cows were administered carprofen at a dose of 1.4 mg/kg body weight via subcutaneous injection in the neck. Muscle, liver, fat and kidney samples were collected from cows at 12 h, 3 d, 7 d, 14 d and 21 d time points after a single administration. After processing the tissue samples, the content of carprofen was detected by ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The methodological results showed that the method had a good linear relationship within the addtion range of 200~2000 ng/mL, and the correlation coefficient r≥0.99. The average recoveries of carprofen in different tissues were between 87.79% and 113.83%, the intra batch and inter batch coefficients of variation were less than 15%. Calculated by the calculation software WT1.4, the withdrawal periods of muscle, liver, kidney, and fat were 7.75 d, 10.04 d, 10.25 d, and 7.54 d, respectively. The residue of the injection site at 21 d was below the MRL in muscle.Therefore, carprofen injection should be used in dairy cows according to the recommended dosing regimen, and it is recommended to set the withdrawal period at 21 d.