疏肝散对慢性肝炎大鼠模型的疗效研究

Study on the efficacy of Shugansan on a rat model of chronic hepatitis

关键词:  疏肝散  四氯化碳  慢性肝炎  SD大鼠

Key words:  Shugansan  Carbon tetrachloride  Chronic hepatitis  SD rats

摘要:

本研究旨在探究中药复方制剂疏肝散对慢性肝炎大鼠的疗效作用。试验选取60只雄性SD大鼠，按体重随机分为空白对照组、模型对照组、疏肝散低、中、高剂量组和阳性对照组，每组10只。除空白对照组外，其余各组按1.0mL/kg皮下注射40%四氯化碳(CCl4)溶液，空白对照组皮下注射等体积的大豆油，2次/周，连续10周；同时，从实验第一天起灌胃给药，各给药组按10mL/kg灌胃给予相应剂量药液，空白对照组和模型对照组灌胃等体积纯水，每天给药1次，连续10周。试验期间观察大鼠食欲、状态、精神情况，末次给药后禁食12h后采血、解剖。检测各组大鼠血清中谷丙转氨酶（ALT）和谷草转氨酶（ALT）、Ⅲ型胶原蛋白(Col-Ⅲ)和Ⅳ型胶原蛋白(Col-Ⅳ)、白介素-1β(IL-1β)和白介素-6(IL-6)的表达情况，计算肝脏脏器系数，观察肝脏组织病理情况。结果显示，与空白对照组比较，模型对照组血清中ALT、ALT、Col-Ⅲ、Col-Ⅳ、IL-1β、IL-6、肝脏脏器系数均明显或显著升高(P<0.05或者P<0.01)，肝脏组织空泡变形明显；与模型对照组比较，疏肝散各剂量组和阳性对照组均能明显或显著降低ALT、AST、Col-Ⅲ、Col-Ⅳ、IL-1β、IL-6、肝脏脏器系数和缓解肝脏组织病变程度。结果提示，中药复方制剂疏肝散对慢性肝炎大鼠模型有明显的疗效作用。

Abstract:

The purpose of this study was to investigate the efficacy of the traditional Chinese medicine compound preparation Shugansan on rats with chronic hepatitis.Sixty male SD rats were randomly divided into a blank control group,a model control group,a low,medium,and high dose group of Shugan San,and a positive control group,with 10 rats in each group.Except for the blank control group,the other groups were injected subcutaneously with 40% tetrachloromethane(CCl4) solution at a dose of 1.0 mL/kg twice a week for 10 weeks.The blank control group was injected with an equal volume of soybean oil.Meanwhile,the rats in the treatment groups were given oral doses of the corresponding concentrations of the drug solution by gavage at a dose of 10 mL/kg once a day for 10 weeks.The blank control group and the model control group were given equal volumes of pure water by gavage once a day for 10 weeks. During the experiment, the rats" appetite, state, and mental condition were observed.Blood samples was collected and the rats were dissected after a 12-hour fast following the last dose.The levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST),type III collagen(Col-III)and type IV collagen(Col- IV),nterleukin-1β(IL-1β)and interleukin-6(IL-6),liver organ coefficient, and histopathological changes in the liver were detected and calculated.The results showed that compared with the blank control group,the model control group had significantly increased levels of serum ALT,AST,Col-III,Col-IV,IL-1β,IL-6,liver organ coefficient,and histopathological score(P <0.05orP <0.01).Compared with the model control group,all dosage groups of Shugan San and the positive control group could significantly or markedly reduce ALT,AST,Col-Ⅲ,Col-Ⅳ,IL-1β,IL-6,liver organ coefficient and pathological tissue score. The results suggested that the TCM compound preparation Shugansan had a significant curative effect on the rat model of chronic hepatitis.