| 摘要: |
| 目的：建立板蓝根注射液尿苷、鸟苷、(R,S)-告依春、腺苷含量检测方法及指纹图谱研究。方法：采用高效液相色谱法,色谱柱:Eclipse Plus C18柱(4.6*250mm, 5μm),检测波长250nm,以甲醇(A)-水(B)为流动相,梯度洗脱(0-3min,95%B；3-20min,95%B-90%B；20-40min,90%B-30%B；40-42min,30%B-95%B；42-50min,95%B),流速为1.0mL/min,柱温箱为30℃,进样量10μL。结论:该实验操作简单、快捷,结果准确,适用板蓝根注射液尿苷、鸟苷、(R,S)-告依春、腺苷含量的测定,建立指纹图谱,对板蓝根注射液进行质量评估。 |
| 关键词: 板蓝根注射液 尿苷 鸟苷 (R,S)-告依春 腺苷 指纹图谱 |
| DOI： |
| 投稿时间：2023-11-08修订日期：2024-04-09 |
| 基金项目:贵州省科技厅项目“常用中兽药制剂质量控制关键技术示范与推广”(合同编号：黔科合成果[2021]一般039)、“畜禽用抗菌中药复方制剂和饲用天然植物提取物的研究与示范”(合同编号:黔科合成果[2022]一般 008);贵州省农业农村厅项目“贵州省现代肉牛产业技术体系建设”(GZCYTX-0302) |
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| Study on the Characteristic Graph and Quality Correlation ofBanlangen Injection |
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| Abstract: |
| Objective: To establish a method for detecting the content of uridine, guanosine, (R,S)-epigoitrin, and adenosine in Banlangen injection and to study their fingerprints. Method: High performance liquid chromatography was used, with Eclipse Plus C18 column (4.6 * 250mm, 5 μ m) Detection wavelength: 250nm, using methanol (A) - water (B) as the mobile phase, gradient elution (0-3min, 95% B; 3-20min, 95% B-90% B; 20-40min, 90% B-30% B; 40-42min, 30% B-95% B; 42-50min, 95% B), flow rate: 1.0mL/min, column temperature chamber: 30 ℃, injection volume: 10 μ L. Conclusion: The experimental operation is simple, fast, and the results are accurate. It is suitable for the determination of uridine, guanosine, (R,S)-epigoitrin e, and adenosine content in Banlangen injection, and the establishment of a fingerprint for the quality evaluation of Banlangen injection. |
| Key words: Banlangen injection Uridine Guanosine (R,S)-Epigoitrin Adenosine Fingerprint |
