HPLC法同时测定茵栀解毒颗粒中3种有效成分的研究

Research on the Simultaneous Determination of Three Active Ingredients in Gardenia Jasminoides Granules by HPLC

关键词:  茵栀解毒颗粒  绿原酸  栀子苷  黄芩苷  高效液相色谱法

Key words:  yinzhi jiedu granule  chlorogenic acid  gardenia glycosides  baicalin  high performance liquid chromatography

摘要:

为了建立同时测定中兽药茵栀解毒颗粒中绿原酸、栀子苷和黄芩苷的方法，研究采用高效液相色谱法，选用C18色谱柱（250 mm×4.6 mm，5 μm），乙腈和0.05%磷酸溶液为流动相，流速1.0 mL/min，进样量10 μL，柱温为30 ℃，检测波长为230 nm。结果表明，栀子苷在3.110～155.5 μg/mL、黄芩苷在1.897～94.86 μg/mL、绿原酸在2.478～123.9 μg/mL浓度范围内线性关系良好，相关系数均大于0.999，平均加样回收率分别为97.1%、96.2%和95.3%。该试验操作简便、快捷、定性、定量准确，能满足中兽药茵栀解毒颗粒中3种有效成分同时检测的需要。

Abstract:

In order to establish a method for the simultaneous determination of chlorogenic acid, gardenia glucoside and baicalin in the Chinese veterinary medicine gardenia antidotes, a C18 column (250 mm×4.6 mm, 5 μm) with acetonitrile and 0.05% phosphoric acid as the mobile phases was used, the flow rate was 1.0 mL/min, the injection volume was 10 μL, the column temperature was 30 ℃ and the detection wavelength was 230 nm. The linearity of the method was good in the range of 3.110 μg/mL to 155.5 μg/mL for gardenia glucoside, 1.897 μg/mL to 94.86 μg/mL for baicalin and 2.478 μg/mL to 123.9 μg/mL for chlorogenic acid, with the correlation coefficients greater than 0.999. The average recoveries were 97.1%, 96.2% and 95.3%, respectively. The test is simple, rapid, qualitative and quantitative, and can meet the needs of the simultaneous determination of the three active ingredients in the Chinese veterinary medicine gardenia antidote granules.