1株鹦鹉热衣原体的冻干、鉴定和分子分型研究

Lyophilization, Identification and Molecular Classification of a Chlamydia psittaci

关键词:  鹦鹉热衣原体  冻干  鉴定  分型

Key words:  Chlamydia psittaci  lyophilization  identification  classification

摘要:

从国家兽医微生物菌（毒）种保藏中心领取1支鹦鹉热衣原体CVCC2410进行冻干和鉴定。将衣原体经卵黄囊接种SPF鸡胚进行复壮和传代，收获卵黄囊膜研磨后分装、冻干，并对冻干衣原体进行了无菌检验、真空度测定、染色特性和形态观察、特异性检验、16S rRNA鉴定、分子分型、衣原体含量测定。结果表明冻干衣原体CVCC2410 2203为C型鹦鹉热衣原体，含量为10^5.50ELD₅₀/0.2mL。本研究完善了该株衣原体的信息，为制定鹦鹉热衣原体的入库标准奠定基础。

Abstract:

A Chlamydia psitsiti CVCC2410 was obtained from the National Center for Veterinary Culture Collection for lyophilization and identification. Chlamydia psittaci was inoculated into SPF chicken embryos through the yolk sac for recovery and passage, and the harvested yolk sac membrane was ground, bottled and then lyophilized. The freeze-dried Chlamydia psittaci was tested for sterility, vacuum measurement, staining characteristics and morphology observation, specificity test, 16S rRNA identification, molecular typing and content determination. The results showed that CVCC2410 2203 was C-type Chlamydia psittaci with a titer of 10^5.50ELD₅₀/0.2mL. This study provided more the information about this strain, and laid the foundation for the formulation of storage standards for Chlamydia psittaci.