规模化养鸡场A型产气荚膜梭菌的分离鉴定及其对海南霉素敏感性的测定

Isolation and identification of Clostridium perfringens from type A in large-scale chicken farm and determination of hainanmycin susceptibility

关键词:  海南霉素  产气荚膜梭菌  抑菌试验

Key words:  Hainanmycin  Clostridium perfringens  Bacteriostatic test

摘要:

为探究海南霉素对产气荚膜梭菌的敏感性，在某大型养鸡场采集了209份鸡肠道样本，采用选择性培养基和MALDI-TOF质谱仪微生物鉴定仪鉴定，并对鉴定的菌株进行分型。采用琼脂稀释法测定了海南霉素、恩拉霉素、盐霉素、丁酸甘油酯对临床分离的产气荚膜梭菌的抗菌活性，以期为当前限抗禁抗后养殖场选择合适的药物控制产气荚膜梭菌提供理论依据。试验结果表明：(1) 共分离出61株产气荚膜梭菌，分离率为29.2%；(2) 通过多重PCR对所分离的产气荚膜梭菌进行分型，结果分离株均为A型产气荚膜梭菌；(3) 4种药物均有不同程度的抑菌效果，其中海南霉素与恩拉霉素对鸡源产气荚膜梭菌的抑菌效果最好，且MIC值范围均为0.004 μg/ml~0.06 μg /mL。由此证明，海南霉素具有很好的抗梭菌效果。

Abstract:

In order to explore the sensitivity of Hainanmycin to Clostridium perfringens, 209 intestinal samples of chickens were collected from a large poultry farm and identified by selective medium and MALDI-TOF mass spectrometer, and the strains were classified. The antimicrobial activities of hainanmycin, enramycin, salinomycin and tributyrin to Clostridium perfringens were determined by agar plate dilution method, in order to provide a theoretical basis for the selection of suitable drugs to control Clostridium perfringens after the current restriction and prohibition. The results showed as follows: (1) A total of 61 strains of Clostridium perfringens were isolated, with a separation rate of 29.2%; (2) The isolated Clostridium perfringens were classified by multiple PCR, and the results showed that all the isolated Clostridium perfringens were TYPE A. (3) All the four drugs had different degrees of antibacterial effect, among which hainanmycin and enramycin had the best antibacterial effect on Clostridium perfringens from chicken, and their MIC values ranged from 0.004 μg/ mL to 0.06 μg/ ml.This proves that Hainanmycin has a good anti clostridium effect.