不同佐剂制备的猪伪狂犬基因缺失标记灭活疫苗免疫效果的比较研究

Comparative Study on the Immune Effect of Pig pseudorabies gene deletion marker inactivated vaccine Prepared with Different Adjuvants

关键词:  佐剂  猪伪狂犬灭活疫苗  稳定性  安全性  免疫效力  

Key words:  Ajuvant  Pig pseudorabies inactivated vaccine  Stability  Safety  Immune efficacy

摘要:

佐剂的剂型对疫苗稳定性、安全性及免疫效力均有一定的影响。为筛选优质佐剂以提高猪伪狂犬基因缺失标记灭活疫苗（JS 2012ΔgE株）的免疫效果，分别使用水包油包水佐剂（ISA206）、水包油佐剂（ISA28VG）、油包水佐剂（ISA70VG）及水佐剂（Gel02）制备疫苗，并检测各组疫苗理化特性、安全性及免疫效力。结果显示：4种佐剂制备的猪伪狂犬灭活疫苗的理化特性、安全性均达到质量标准；免疫效力比较中，ISA70VG组相比其他免疫组具有较高的抗体水平且持续时间较长，其次为ISA206组；攻毒后, ISA70VG组和ISA206组无任何临床症状及病变。综合安全性及免疫效力，ISA206佐剂及ISA70VG更适合作为制备猪伪狂犬基因缺失标记灭活疫苗（JS 2012ΔgE株）的佐剂。

Abstract:

The dosage form of adjuvant has a certain impact on the stability, safety and immune efficacy of vaccine. In order to select high-quality adjuvants to improve the immune effect of the pig pseudorabies gene deletion inactivated vaccine(JS 2012ΔgE strain), we prepared 4 kinds of vaccines with water in oil-in-water adjuvant (ISA206), Oil-in-water adjuvant (ISA28VG), water-in-oil adjuvant (ISA70VG) and water adjuvant (Gel02) respectively, and detected physical and chemical properties, safety and immune efficacy of the vaccine. The results showed that the physical and chemical properties and safety of 4 kinds of inactivated vaccines all reached the quality standard; in the comparison of immune efficacy, the ISA70VG group had higher antibody levels and longer duration compared to other immune groups, followed by the ISA206 group; after the challenge, the ISA70VG group and ISA206 group showed no clinical symptoms or lesions. Considering the safety and immune efficacy, ISA206 adjuvant and ISA70VG are more suitable as adjuvants for the preparation of porcine pseudorabies gene deletion marker inactivated vaccine (JS 2012ΔgE strain).