基于网络药理学探讨黄芩素治疗猪链球菌脑膜炎的分子机制

Molecular Mechanism of Baicalein in Treating Streptococcus Suis Meningitis Based on Network Pharmacology

关键词:  黄芩素  猪链球菌脑膜炎  网络药理学

Key words:  Baicalein  Streptococcus Suis Meningitis  Network Pharmacology

摘要:

为探讨黄芩素治疗猪链球菌脑膜炎的分子机制，应用网络药理学的方法，在Pubchem和SwissTarget Prediction数据库获取黄芩素490个潜在作用靶点，并在NCBI等平台中检索猪链球菌脑膜炎的靶点。运用STRING数据库构建“中药-疾病”共同靶点的PPI网络图；利用DAVID数据库进行GO功能注释和KEGG通路富集分析，并使用Cytoscape3.8.2软件构建“中药-靶点”通路图。结果显示黄芩素和猪链球菌脑膜炎有9个共同靶点(MMP2、SYK、FN1、MAPK14、HSPCB、CASP3、CCL2、APOD、EGFR)，其中MMP2、SYK和FN1是脑损伤的核心靶点。GO富集分析发现，黄芩素可能通过影响细胞凋亡的调控过程、细胞外间隙、细胞氧化应激的过程缓解猪链球菌脑膜炎的症状；KEGG通路主要富集在IL-17信号通路、MAPK信号通路等41条相关通路。基于网络药理学的结果，本研究探讨了黄芩素潜在治疗链球菌引起的脑膜炎的作用，推测该中药成分可以作为开发抗猪链球菌疾病的先导化合物，为开发治疗猪链球菌病的药物奠定研究基础。

Abstract:

To analyze the molecular mechanism of baicalein in the treatment of Streptococcus suis meningitis, network pharmacology was used to analysis the mechanism. 490 targets for the therapeutic actions of baicalein were obtained from PubChem and Swiss Target Prediction, and the potential disease targets of Streptococcus suis meningitis were obtained from NCBI. The common target PPI network map of “Drug-Disease” was using the STRING database. GO and KEGG pathway enrichment analysis was performed using the DAVID database, and “Drug-Target” pathway network map was constructed by using Cytoscape 3.8.2 software. Results showed that 9 common targets were obtained of Streptococcus suis and baicalein, including MMP2、SYK、FN1、MAPK14、HSPCB、CASP3、CCL2、APOD、EGFR, and of which MMP2、SYK、FN1 were the key targets of brain injury. GO enrichment analysis revealed that baicalein may alleviate symptoms of Streptococcus suis meningitis by affecting the regulatory processes of apoptosis, extracellular space, and cellular response to reactive oxygen species. KEGG pathway enrichment screening yielded 41 related pathways including IL-17 signaling pathway and MAPK signaling pathway. Based on network pharmacology, the potential effect of baicalein in the treatment of streptococcal meningitis was studied in this paper, which supposed that baicalein could be used as a lead compound for the development of anti-streptococcal disease and lay the research foundation for the development of drugs for the treatment of streptococcal disease in pigs.