鸭坦布苏病毒自然感染病例的诊断与病理学观察

Diagnosis and Pathological Observation of Duck Tembusu Virus Naturally Infected Cases

关键词:  鸭  坦布苏病毒  自然感染  病理组织

Key words:  Duck  TMUV  Natural infection  histopathology

摘要:

为探究DTMUV(Duck tembusu virus, DTMUV)在贵州省的发病情况,对疑似DTMUV感染肉种鸭进行剖检,观察发病鸭内脏器官病变。取内脏组织进行RT-PCR检测确诊为DTMUV感染,将病料处理后接种BHK-21细胞,进行RT-PCR鉴定和透射电镜观察,并将病料组织制作病理切片。结果显示,剖检肝脏肿大,色泽偏黄色,心脏和肾脏表面均有不同大小出血点,脑组织出血、水肿。将病料接种于BHK-21细胞培养60 h可见明显细胞病变,采用RT-PCR扩增后琼脂糖凝胶电泳可见与预期大小相符的目的条带,经透射电镜观察可见病毒颗粒。将病料制作切片并进行HE染色,可见脑组织非化脓性脑炎,脾脏淋巴细胞减少,肝细胞变性坏死。综上所述,DTMUV在贵州省确有发生,在养殖中需加强对该病的防控。

Abstract:

In order to explore the incidence of Duck tembusu virus (DTMUV) in Guizhou Province, necropsy was performed on broiler ducks suspected of DTMUV infection, and the internal organs lesions of the infected ducks were observed. The visceral tissue was detected by RT-PCR and confirmed to be DTMUV infection. The diseased material was inoculated with BHK-21 cells, identified by RT-PCR and observed by transmission electron microscope. Pathological sections were made of the diseased material. The results showed that the necropsy revealed that the liver was enlarged and yellowish in color, the heart and kidney surface had bleeding spots of different sizes, and the brain tissue was bleeding and edema. The disease material was inoculated into BHK-21 cells and cultured for 60 hours, and obvious cytopathic changes were observed. After amplification by RT-PCR, agarose gel electrophoresis showed the target band with the expected size, and virus particles were observed by transmission electron microscope. The diseased material was sliced and stained with HE. The brain tissue was non-suppurative encephalitis, the spleen lymphocytes decreased, and the liver cells were degenerated and necrotic. To sum up, DTMUV does occur in Guizhou Province, and it is necessary to strengthen the prevention and control of the disease in breeding.