猪丹毒杆菌SpaA优势表位的串联表达及其免疫效果分析

Construction of Erysipelas Subunit Vaccine with Tandem SpaA Dominant Epitopes

关键词:  串联  SpaA  优势表位  丹毒  疫苗  免疫原性  攻毒保护  猪

Key words:  tandem  SpaA  dominant epitopes  Erysipelas  vaccine  immunogenicity  protection against challenge  pig

摘要:

目的 构建串联SpaA优势表位的丹毒亚单位疫苗，并对其免疫原性进行研究。方法 将丹毒杆菌表面保护抗原A优势表位串联表达，提取并经亲和层析纯化获得重组蛋白，制备亚单位疫苗，免疫小鼠和猪，21日后攻毒。结果 成功制备串联SpaA优势表位的丹毒亚单位疫苗，2μg重组蛋白可为小鼠抵抗1000MLD强毒攻击提供100%保护，100μg重组蛋白可为猪抵抗1MLD强毒攻击提供100%保护，可为猪抵抗2MLD强毒攻击提供67%保护。结论 串联SpaA优势表位的丹毒亚单位疫苗具有良好的免疫原性，高于商品化疫苗保护标准。

Abstract:

Abstract:Objective The purpose of this study was to construct a subunit vaccine of Erysipelas with the dominant epitopes of SpaA of Erysipelothrix erysipelas in tandem,and to study its immunogenicity.Methods The dominant epitopes of surface protection antigen A of Erysipelothrix erysipelas was expressed in tandem,and the recombinant protein was extracted and purified by affinity chromatography.The subunit vaccine was prepared and immunized mice and pigs which was challenged 21 days later.Results The Erysipelas subunit vaccine with the dominant epitopes of SpaA in tandem was successfully prepared.2μg recombinant protein can provide 100% protection for mice against 1000MLD virulent attack.100μg recombinant protein can provide 100% protection against the attack of 1MLD virulent attack and 67% protection against the attack of 2MLD virulent attack for pigs.Conclusion The Erysipelas subunit vaccine with the dominant epitope of SpaA in tandem has good immunogenicity, which is higher than the protection standard of commercial vaccine.