复方中药治疗鸡实验性镉中毒的作用机理

Mechanism of compound chinese medicine in treating experimental cadmium poisoning in chickens

关键词:  镉中毒  复方中药  鸡  抗氧化  镉蓄积

Key words:  cadmium poisoning  compound chinese medicine  chicken  antioxidant  cadmium accumulation

摘要:

目的：为研究复方中药治疗鸡实验性镉中毒的作用机理。方法：试验选取90只鸡随机分为3组(空白对照组、阳性组、中药治疗组),每组30只。空白组饲喂基础日粮,阳性组饲喂基础日粮+40.31 mg/kg氯化镉,中药治疗组饲喂基础日粮+40.31 mg/kg氯化镉+4.7 g/kg复方中药,试验期为90d。在试验第30d、60d、90d,每组随机取10只鸡,测定体重,采血测血清中丙二醛(MDA)、一氧化氮(NO)的含量,超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、一氧化氮合酶(NOS)的活性,取心脏、肝脏、脾脏、肺脏、肾脏、跖骨检测其镉含量,90d时另取部分组织器官进行固定制作切片。结果：(1)阳性组鸡体重均显著低于空白对照组鸡体重(P<0.05),中药治疗组鸡体重显著高于阳性组鸡体重(P<0.05)；(2)中药治疗组能明显缓解镉引起心脏、肝脏、脾脏、肺脏、肾脏、跖骨炎性细胞浸润,出血等病变；(3)阳性组血清中SOD、GSH-Px、NOS活性NO含量显著低于空白对照组(P＜0.05),MDA含量显著高于空白对照组(P＜0.05)；中药治疗组SOD、GSH-Px、NOS活性NO含量显著高于阳性组(P＜0.05),MDA含量显著低于阳性组(P＜0.05)；(4)阳性组各组织器官镉含量显著高于空白对照组(P＜0.05),中药治疗组各组织器官镉含量显著低于阳性组(P＜0.05)。讨论：复方中药能降低鸡组织器官镉蓄积,增强实验性镉中毒鸡的抗氧化能力,缓解镉引起鸡组织器官的损伤。

Abstract:

In order to study the mechanism of compound Chinese medicine in treating experimental cadmium poisoning in chickens, 90 chickens were randomly divided into three groups (conventional group, positive group and Chinese medicine treatment group), 30 chickens in each group.. The conventional group was fed with basic diet, the positive group was fed with basic diet + 40.31mg/kg dose of CdCl2, and the traditional Chinese medicine treating group was fed with basic diet + 40.31mg/kg dose of CdCl2+ 4.7g/kg traditional Chinese medicine for 90 days. On the 30th, 60th and 90th days of the experiment, 10 chickens in each group were randomly taken. The body weight and the contents of MDA, SOD, GSH-Px, NO and NOS in serum were measured. After death, the contents of cadmium in heart, liver, spleen, lung, kidney and leg bone were measured. At 90th day, took parts of heart、liver、spleen、lung、kidney、leg bone and fixed them in the related fixative to prepare for making slices. Results:(1)The body weight of the cadmium group was significantly lower than that of the blank control group (P<0.05), and that of the traditional Chinese medicine treatment group was significantly higher than that of the cadmium group (P<0.05);(2)The traditional Chinese medicine treatment group can obviously alleviate the inflammatory cell infiltration and bleeding of the heart, liver, spleen, lung, kidney and metatarsal bone caused by cadmium;(3)The content of SOD, GSH Px, NOS activity and NO in the positive group was significantly lower than that in the blank control group (P<0.05), while the content of MDA was significantly higher than that in the blank control group (P<0.05); The content of SOD, GSH Px and NOS activity NO in the treatment group was significantly higher than that in the positive group (P<0.05), while the content of MDA was significantly lower than that in the positive group (P<0.05);(4)The cadmium content in the positive group was significantly higher than that in the conventional group (P<0.05). The cadmium content in tissues and organs of the Chinese medicine treatment group was significantly lower than that of the positive group (P＜0.05). Discussion:The compound Chinese medicine can reduce the accumulation of cadmium in chicken tissues and organs, enhance the antioxidant capacity of experimental cadmium poisoned chickens, and alleviate the damage of chicken tissues and organs caused by cadmium.