马丁琼脂培养基质量控制标准研究

Study on Quality Control Standard of Martin Agar Medium

关键词:  马丁琼脂培养基  活菌计数  参考品  适应性  促生长能力

Key words:  Martin agar medium  live bacteria count test  reference  adaptability  growth-promoting ability

摘要:

为建立马丁琼脂培养基质量控制标准，系统评价马丁琼脂培养基适应性和促生长能力，运用多杀性巴氏杆菌、丹毒杆菌和链球菌类活疫苗活菌计数参考品，通过活菌计数统计分析，对新鲜马丁琼脂、干粉马丁琼脂或改良马丁琼脂进行适应性和促生长能力系统测试。结果显示：新鲜马丁琼脂、干粉马丁琼脂和改良马丁琼脂均对多杀性巴氏杆菌、丹毒杆菌、链球菌的适应性和促生长能力差异显著，需同时选择巴氏杆菌类、丹毒杆菌类、链球菌类活疫苗计数参考品评价马丁琼脂培养基的适应性和促生长能力，表明活菌计数参考品用于建立马丁琼脂质量控制标准是可行的。

Abstract:

In order to evaluate the adaptability and growth promoting ability of Martin agar medium systematically, and establish quality control standard of Martin agar medium. This study used Pasteurella multocida, erysipelas and Streptococcus live vaccine viable count reference to test the adaptability and growth promoting ability of fresh Martin agar, dry powder Martin agar and modified Martin agar through the statistical analysis of viable count . The results show that the adaptability and growth ability is very different among Pasteurella multocida, erysipelas and Streptococcus to Fresh Martin agar, Martin agar powder and modified Martin agar. It is necessary to choose Pasteurella multocida, erysipelas and Streptococcus live vaccine viable count reference at the same time, to evaluate the adaptability and evaluation ability of Martin agar medium, shows that it is feasible to establish an standard index for quality control of Martin AGAR with viable count reference.