HPLC法同时测定杜仲叶提取物中5种成分含量

Simultaneous Determination of Five Compoundsin Eucommiae Folium Extract by HPLC

关键词:  杜仲叶提取物  5种成分  HPLC

Key words:  Eucommiae Folium extract  Five compounds  HPLC

摘要:

建立同时测定杜仲叶提取物中5种成分含量的HPLC方法，色谱柱为岛津 WondaCract ODS2 C18（4.6mm*150mm，5μm）；流动相甲醇-0.4%磷酸水溶液，梯度洗脱，流速1mL·min-1；柱温30℃；进样量10μL；京尼平苷酸、车叶草苷检测波长为238nm，桃叶珊瑚苷、绿原酸、芦丁的检测波长分别为200nm、327nm、254nm。结果表明，5种成分在检测范围内线性关系良好（R2≥0.9999），平均加样回收率为98.4%～101.0%，RSD 1.06%～1.50%，10批杜仲叶提取物中5种成分含量的平均值分别为25.83、40.21、13.12、64.72、11.03 mg/g，本方法稳定可靠，重复性好，可对杜仲叶提取物样品中5种指标成分的含量进行同时测定。

Abstract:

To establish a method for the simultaneous determination of five compounds in Eucommiae Folium extract by HPLC, the quantitative analysis was carried out on a Shimadzu WondaCract ODS2 C18 column (4.6mm*150mm，5μm) with mobile phase of methanol (A)- 0.4% phosphoric acid aqueous solution (B) for gradient elution.The flow rate was 1.0 mL·min-1. The column temperature was maintained at 30 ℃. The injection volume was 10μL. The detection wavelength of geniposidic acid and asperuloside was 238nm. The detection wavelength of aucubin, chlorogenic acid and rutin was 200nm, 327nm and 254nm, respectively. The results show that the linear relationship of the five compounds was good (R2≥0.9999). The average recovery was 98.4%~101.0%, RSD 1.06%~1.50%. The average contents of five components in 10 batches of Eucommiae Folium extracts were 25.83, 40.21, 13.12, 64.72 and 11.03 mg/g, respectively. The method is stable, reliable and reproducible. It can simultaneous determine the contents of five target ingredients in Eucommiae Folium extract sample.