猪传染性胃肠炎、猪流行性腹泻二联活疫苗耐热保护技术的研究

Study on Heat-resistant Protection Technology of Swine Transmissible Gastroenteritis and Porcine Epidemic Diarrhea combined live vaccine

关键词:  猪传染性胃肠炎  猪流行性腹泻  二联活疫苗  耐热保护技术

Key words:  porcine infectious gastroenteritis  porcine epidemic diarrhea  combined live vaccine  heat-resistant protection technology

摘要:

疫苗保护剂及其冻干工艺是影响疫苗效果的重要因素，对疫苗保存、运输及免疫效果有很大影响。为提高疫苗质量，在常规保护剂（明胶、蔗糖）基础上添加卡波姆、聚乙二醇和聚乙烯吡咯烷酮等基质，组成新型保护剂，分别在病毒液保存和疫苗冻干过程添加，并评价其病毒保护效果。结果表明：在半成品病毒液储存时加入10%新型保护剂，可使半成品保存期由原来的2个月延长至12个月。采用新型保护剂制备的猪传染性胃肠炎、猪流行性腹泻二联活疫苗，溶解性显著优于常规保护剂活疫苗，保存期由原来-20 ℃保存18个月提高到2～8 ℃保存36个月，且病毒活性及攻毒保护效力均优于原工艺产品，实现了储运过程中疫苗的冷藏储运，提升了疫苗的应用效果。

Abstract:

Vaccine protective agent and its lyophilized process are important factors affecting vaccine efficacy, which have great influence on vaccine preservation, transportation and immune efficacy. In order to improve the quality of the vaccine, carbomer, polyethylene glycol, polyvinylpyrrolidone and other matrices were added on the basis of the conventional protective agents to form new protective agents, which were added respectively during the process of venom preservation and vaccine lyophilization, and then its protective effect on the virus was evaluated. The results showed that the storage time of semi-finished venom could be extended from 2 months to 12 months by adding 10% new protective agent. The combined live vaccine of porcine infectious gastroenteritis and porcine epidemic diarrhea prepared with the new protective agent has a significantly better solubility than the conventional protective agent live vaccine. At the same time, the shelf life of the vaccine was extended from 18 months at -20 ℃ to 36 months at 2~8 ℃, and the virus activity and virus-fighting protective efficacy are better than the original process products, which realizes the cold storage and transportation of vaccines during storage and transportation, and improved the effectiveness of the vaccine.