噬菌体展示技术及其在单克隆抗体制备中的应用

Phage display technology and its application in monoclonal antibody preparation

关键词:  噬菌体展示  抗体库  单克隆抗体  噬菌体  下一代测序

Key words:  Phage display  antibody library  monoclonal antibody  phage  next-generation sequencing

摘要:

噬菌体展示技术是一种强大的高通量多肽筛选技术,常用于确定靶标蛋白的亲和力与结合位点,并广泛应用于单克隆抗体的制备。随着下一代测序和微流控技术的出现,噬菌体展示技术已成为制备单克隆抗体更为强大和流行的工具。通过对噬菌体展示技术的历史发展与分类、噬菌体展示技术制备单克隆抗体的过程以及其在制备单克隆抗体上的应用进行论述,以期为相关研究人员提供参考。

Abstract:

Phage display technology is an high-throughput peptide screening technique. This method is often used to determine target protein affinity and binding locations. It is also widely used in the preparation of monoclonal antibodies. Phage display technology has become a more potent and popular method for the manufacture of monoclonal antibodies since the introduction of next-generation sequencing and microfluidics. This article discusses the historical development of phage display technology, the classification of phages, the process of preparing monoclonal antibodies by phage display technology, and its application to the preparation of monoclonal antibodies, in order to provide a reference for related researchers.