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牛病毒性腹泻/黏膜病(1型+2型)、牛传染性鼻气管炎、牛副流感(3型)三联灭活疫苗(E2蛋白+C1株+HB01株)的研制与免疫效果评价
赵卓,胡义彬,雷莉辉,武沛泽,武春霞,马岩,张巨峰,王力,江厚生
0
(1.北京生泰尔科技股份有限公司 2. 北京华夏兴洋生物科技有限公司)
摘要:
采用CHO细胞表达牛病毒性腹泻/黏膜病病毒1型(BVDV1)E2蛋白,采用杆状病毒重组表达牛病毒性腹泻/黏膜病病毒2型(BVDV2)E2蛋白,采用MDBK细胞微载体悬浮培养技术培养牛传染性鼻气管炎病毒(IBRV)和牛副流感病毒3型(BPIV3),收获蛋白表达产物和细胞培养物,经纯化、灭活后与605佐剂混合,制备牛病毒性腹泻/黏膜病(1型+2型)、牛传染性鼻气管炎、牛副流感(3型)三联灭活疫苗(E2蛋白+C1株+HB01株)。将疫苗免疫健康易感牛进行免疫效果评价,结果表明该产品免疫效果良好,免疫牛IBRV和BPIV3中和抗体效价均可达到1∶77以上;BVDV1和BVDV2 E2蛋白琼扩抗体效价均可达到1∶32以上;免疫牛攻毒保护率均可达到4/5以上。
关键词:  牛病毒性腹泻/黏膜病  牛传染性鼻气管炎  牛副流感  灭活疫苗  免疫效力
DOI:
投稿时间:2022-05-07修订日期:2022-11-01
基金项目:内蒙古自治区科技重大专项-牛腹泻疾病防控关键技术研发与标准化操作规程示范推广(2020ZD0006)
Development and Immune Effect Evaluation of Bovine Viral Diarrhea/Mucosal Disease (Type 1+Type 2), Infectious Bovine Rhinotracheitis and Bovine Parainfluenza (Type 3) Inactivated Vaccines (E2 Protein+C1 Strain+HB01 Strain)
(1. Beijing Centre Biology Co.,Ltd. 2. Beijing Centrebio Biology Co., Ltd)
Abstract:
In this study, CHO cells were used to express bovine viral diarrhea/mucosal disease virus type 1 (BVDV1)E2 protein, baculovirus recombinant was used to express bovine viral diarrhea/mucosal disease virus type 2(BVDV2) E2 protein, and MDBK cell microcarrier suspension culture technology was used to culture bovine infectious rhinotracheitis virus (IBRV) and bovine parainfluenza virus type 3 (BPIV3). The protein expression products and virus cultures were harvested and mixed with 605 adjuvant after purification and inactivation to prepare inactivated vaccine of bovine viral diarrhea/mucosal disease (type 1+type 2), infectious bovine rhinotracheitis and bovine parainfluenza (type 3) (E2 protein + C1 strain+HB01 strain). The results showed that the immune effect was good, and the titers of IBRV and BPIV3 neutralizing antibodies of immunized cattle were more than 1∶77; The titers of BVDV1 and BVDV2 E2 protein antibody could reach more than 1∶32; The protection rate of immunized cattle against virus could reach more than 4/5.
Key words:  bovine viral diarrhea/mucosal disease  infectious bovine rhinotracheitis  bovine parainfluenza  inactivated vaccine  immune efficacy

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