牛病毒性腹泻/黏膜病(1型+2型)、牛传染性鼻气管炎、牛副流感(3型)三联灭活疫苗(E2蛋白+C1株+HB01株)的研制与免疫效果评价

Development and Immune Effect Evaluation of Bovine Viral Diarrhea/Mucosal Disease (Type 1+Type 2), Infectious Bovine Rhinotracheitis and Bovine Parainfluenza (Type 3) Inactivated Vaccines (E2 Protein+C1 Strain+HB01 Strain)

关键词:  牛病毒性腹泻/黏膜病  牛传染性鼻气管炎  牛副流感  灭活疫苗  免疫效力

Key words:  bovine viral diarrhea/mucosal disease  infectious bovine rhinotracheitis  bovine parainfluenza  inactivated vaccine  immune efficacy

摘要:

采用CHO细胞表达牛病毒性腹泻/黏膜病病毒1型（BVDV1）E2蛋白，采用杆状病毒重组表达牛病毒性腹泻/黏膜病病毒2型（BVDV2）E2蛋白，采用MDBK细胞微载体悬浮培养技术培养牛传染性鼻气管炎病毒（IBRV）和牛副流感病毒3型（BPIV3），收获蛋白表达产物和细胞培养物，经纯化、灭活后与605佐剂混合，制备牛病毒性腹泻/黏膜病(1型+2型)、牛传染性鼻气管炎、牛副流感(3型)三联灭活疫苗(E2蛋白+C1株+HB01株)。将疫苗免疫健康易感牛进行免疫效果评价，结果表明该产品免疫效果良好，免疫牛IBRV和BPIV3中和抗体效价均可达到1∶77以上；BVDV1和BVDV2 E2蛋白琼扩抗体效价均可达到1∶32以上；免疫牛攻毒保护率均可达到4/5以上。

Abstract:

In this study, CHO cells were used to express bovine viral diarrhea/mucosal disease virus type 1 （BVDV1）E2 protein, baculovirus recombinant was used to express bovine viral diarrhea/mucosal disease virus type 2（BVDV2） E2 protein, and MDBK cell microcarrier suspension culture technology was used to culture bovine infectious rhinotracheitis virus （IBRV） and bovine parainfluenza virus type 3 (BPIV3). The protein expression products and virus cultures were harvested and mixed with 605 adjuvant after purification and inactivation to prepare inactivated vaccine of bovine viral diarrhea/mucosal disease (type 1+type 2), infectious bovine rhinotracheitis and bovine parainfluenza (type 3) (E2 protein + C1 strain+HB01 strain). The results showed that the immune effect was good, and the titers of IBRV and BPIV3 neutralizing antibodies of immunized cattle were more than 1∶77; The titers of BVDV1 and BVDV2 E2 protein antibody could reach more than 1∶32; The protection rate of immunized cattle against virus could reach more than 4/5.