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鸡球虫病活疫苗不同卵囊计数方法的比较
张媛,任小侠,王秀丽,王楠,李建,张一帜,刘元杰,褚智菲,宋运娇,于慧娟,赵金羽,高彬,刘洋,王晨旭,张慧,石宗昌,梁正鹏,刘丽丹,吴金香,罗玉峰,李俊平
0
(中国兽医药品监察所)
摘要:
为了解鸡球虫活疫苗不同卵囊计数方法对检测结果的影响,分别采用血细胞计数板4角方格法和9方格法以及麦氏计数板法对3种鸡球虫活疫苗各进行了6次卵囊计数,然后采用t检验和Z比分值方法比较筛选出最适宜的计数方法,并对检测方法进行了验证和变异系数统计。结果显示:采用每批检品抽检1瓶,通过血细胞计数板4角方格法重复计数6次,去除最大值和最小值后取平均值作为该瓶检品卵囊计数结果,如果首次检验结果不符合规定可重检一次的方法,人员误差最小。该方法提高了鸡球虫活疫苗卵囊计数结果的可重复性和准确性,增强了疫苗质量控制的可靠性。
关键词:  球虫  卵囊计数  比较
DOI:
投稿时间:2022-04-26修订日期:2022-10-28
基金项目:
Comparison of Different Oocyst Counting Methods of Live Chicken Coccidiosis Vaccine
(China Institute of Veterinary Drug Control)
Abstract:
In order to find the influence on the test results of different oocyst counting results of live chicken coccidiosis vaccine, the counting chamber 4-corner square method, the counting chamber 9 square method and McMaster counting method were used in oocyst counting 6 times each. Then t-test and Z-ratio score method were used to select the most suitable counting method, the test method was verified and the coefficient of variation was counted. The results showed that each batch was sampled 1 bottle, and counted 6 repeats by the counting chamber 4-corner square method. After removing the maximum value and the minimum value, average value was taken as the oocyst count result, it could be re-inspected once if the result not meet the requirements. This method had the smallest personnel error. The method improves the repeatability and accuracy of the counting results of oocysts of live chicken coccidiosis vaccine, and enhances the reliability of vaccine quality control.
Key words:  Coccidia  Oocyst count  Comparison

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