两种氟苯尼考注射液在鸡体内比较药动学研究

Comparative pharmacokinetic of two florfenicol injections in chicken

关键词:  氟苯尼考  鸡  超高效液相色谱法  比较药动学

Key words:  florfenicol  chicken  UPLC  comparative pharmacokinetics

摘要:

为比较两种氟苯尼考注射液的药物代谢动力学，本研究选择30只健康鸡随机分为两组，分别单剂量20 mg/kg bw肌内注射受试制剂和参比制剂，于给药后0.167、0.33、0.5、0.75、1、1.5、2、4、6、8、12、24、48 h翼下静脉采集血样。用超高效液相色谱法（UPLC-UV）测定血浆中氟苯尼考的含量，并用WinNonlin 8.1非房室模型计算主要药代动力学参数。结果显示，受试制剂和参比制剂的t1/2分别为（3.39±2.65）和（4.47±3.14）h，Tmax分别为（0.66±0.30）和（0.77±0.30）h，Cmax分别为（7.06±2.35）和（8.24±4.54）μg/mL，AUC0→t分别为（19.05±5.79）和（21.76±6.71）（μg/mL）h，AUC0→∞分别为（20.11±6.36）和（23.04±6.91）（μg/mL）h，MRT分别为（3.25±1.25）和（3.55±0.96）h，相对生物利用度为87.55%。结果表明，虽然二者主要药动学参数无显著性差异（p＞0.05），但受试制剂相对生物利用度较低。

Abstract:

n order to compare the pharmacokinetics of two kinds of florfenicol injections, 30 healthy chickens were randomly divided into two groups, and were given respectively a single dose of test preparation or reference preparation by intramuscular injection (20 mg/kg bw). Blood samples were collected from the inferior wing vein at different times(0.167、0.33、0.5、0.75、1、1.5、2、4、6、8、12、24、48 h). Plasma concentrations of florfenicol were measured by UPLC-UV，and the pharmacokinetic parameters were calculated by WinNonlin 8.1 software ．The main pharmacokinetic parameters of the test preparation and reference preparation were as follows：t1/2 were（3.39±2.65）and（4.47±3.14）h，Tmax were（0.66±0.30）and（0.77±0.30）h；Cmax were（7.06±2.35）and（8.24±4.54）μg/mL；AUC0→t were（19.05±5.79）and（21.76±6.71）（μg/mL）h；AUC0→∞ were（20.11±6.36）and（23.04±6.91）（μg/mL）h；MRT were（3.25±1.25）and（3.55±0.96）h，the relative bioavailability was 87.55%. The results showed that there was no significant difference in pharmacokinetic parameters between the two preparation (P > 0.05), the relative bioavailability of the tested preparation is low.