HPLC法同时测定双葛止泻口服液中绿原酸和葛根素的含量

Determination of Chlorogenic Acid and Puerarin in Shuangge Zhixie Oral Liquid by HPLC

关键词:  绿原酸  葛根素  双葛止泻口服液  高效液相色谱法

Key words:  chlorogenic acid  puerarin  Shuangge Zhixie oral liquid  HPLC

摘要:

[摘 要] 建立了同时测定双葛止泻口服液中绿原酸和葛根素含量的测定方法。采用高效液相色谱（HPLC）法，使用Waters XBridge C18（250 × 4.6 mm，5 μm）色谱柱，流动相为乙腈-0.4%磷酸溶液梯度洗脱；检测波长为315 nm；流速为1.0 mL/min；柱温为30 ℃。结果表明，绿原酸浓度在0.008242~0.1030 mg/mL的范围内，与峰面积线性关系良好（r = 1.0000），平均回收率为99.1%（n = 6），RSD为1.8%；葛根素浓度在0.02296~0.2871 mg/mL的范围内，与峰面积线性关系良好（r = 1.0000），平均回收率为98.7%（n = 6），RSD为1.6%。该方法简单、准确、专属性强，可用于双葛止泻口服液中绿原酸和葛根素的质量控制。

Abstract:

Abstract: The method for determination of the content of chlorogenic acid and puerarin in Shuangge Zhixie Oral Liquid was developed. The analysis was performed on an Waters XBridge C18 column(250 × 4.6 mm, 5 μm). The gradient mobile phase was consisted of acetonitrile-0.4% phosphoric acid solution, and the detection wavelength was 315 nm. The flow rate was 1.0 mL/min and the column temperature was 30 ℃. The linear range of the calibration curves for chlorogenic acid was 0.008242~0.1030 mg/mL (r = 1.0000), and the average recovery was 99.1% (n = 6) with RSD of 1.8%. The linear range of the calibration curves for puerarin was 0.02296~0.2871 mg/mL (r = 1.0000), and the average recovery was 98.7% (n = 6) with RSD of 1.6%. The method is simple and accurate with high specificity, which can be used to determine the content of chlorogenic acid and puerarin in Shuangge Zhixie Oral Liquid.