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HPLC法同时测定双葛止泻口服液中绿原酸和葛根素的含量
周冰,李焕娟,陈帅韬,安卓睿,程婵婵,姚路路,张聪
0
(洛阳惠中兽药有限公司)
摘要:
[摘 要] 建立了同时测定双葛止泻口服液中绿原酸和葛根素含量的测定方法。采用高效液相色谱(HPLC)法,使用Waters XBridge C18(250 × 4.6 mm,5 μm)色谱柱,流动相为乙腈-0.4%磷酸溶液梯度洗脱;检测波长为315 nm;流速为1.0 mL/min;柱温为30 ℃。结果表明,绿原酸浓度在0.008242~0.1030 mg/mL的范围内,与峰面积线性关系良好(r = 1.0000),平均回收率为99.1%(n = 6),RSD为1.8%;葛根素浓度在0.02296~0.2871 mg/mL的范围内,与峰面积线性关系良好(r = 1.0000),平均回收率为98.7%(n = 6),RSD为1.6%。该方法简单、准确、专属性强,可用于双葛止泻口服液中绿原酸和葛根素的质量控制。
关键词:  绿原酸  葛根素  双葛止泻口服液  高效液相色谱法
DOI:
投稿时间:2021-12-13修订日期:2022-09-09
基金项目:
Determination of Chlorogenic Acid and Puerarin in Shuangge Zhixie Oral Liquid by HPLC
(Luoyang Huizhong Veterinary Medicine Co. , Ltd)
Abstract:
Abstract: The method for determination of the content of chlorogenic acid and puerarin in Shuangge Zhixie Oral Liquid was developed. The analysis was performed on an Waters XBridge C18 column(250 × 4.6 mm, 5 μm). The gradient mobile phase was consisted of acetonitrile-0.4% phosphoric acid solution, and the detection wavelength was 315 nm. The flow rate was 1.0 mL/min and the column temperature was 30 ℃. The linear range of the calibration curves for chlorogenic acid was 0.008242~0.1030 mg/mL (r = 1.0000), and the average recovery was 99.1% (n = 6) with RSD of 1.8%. The linear range of the calibration curves for puerarin was 0.02296~0.2871 mg/mL (r = 1.0000), and the average recovery was 98.7% (n = 6) with RSD of 1.6%. The method is simple and accurate with high specificity, which can be used to determine the content of chlorogenic acid and puerarin in Shuangge Zhixie Oral Liquid.
Key words:  chlorogenic acid  puerarin  Shuangge Zhixie oral liquid  HPLC

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