桃金娘果多糖的制备及其免疫增强作用研究

The preparation of Rhodomyrtus tomentosa fruit polysaccharide and its immunoenhancing effect

关键词:  桃金娘果多糖  免疫调节  细胞因子

Key words:  Rhodomyrtus tomentosa fruit polysaccharide  immunomodulatory  cytokines

摘要:

制备桃金娘果多糖并对其单糖组成进行分析，探讨桃金娘果多糖对健康小鼠免疫功能的影响。采用水浴提取法制备桃金娘果多糖，衍生化高效液相色谱法分析单糖成分。选用健康昆明小鼠80只，随机分为空白组、黄芪多糖组[腹腔注射200 μg/（g·BW）]、桃金娘果多糖低、中、高[分别腹腔注射50 μg/（g·BW）、100μg/（g·BW）、200μg/（g·BW）]共5组。空白组每只小鼠腹腔注射等体积的生理盐水，1次/天，连续7天。试验结束后，分别测定小鼠脏器指数（胸腺、脾脏）、血清中IL-1β、IL-2、IL-6、IFN-γ、LZM、POD等免疫指标的含量。结果显示，桃金娘果多糖含量为65.83%，主要由甘露糖、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖、木糖、阿拉伯糖组成。桃金娘果多糖会降低小鼠的胸腺指数，但能显著增强小鼠的脾脏指数；桃金娘果多糖可提高了IL-1β、IL-2、IL-6、IFN-γ血清细胞因子的水平、POD活性和LZM含量。桃金娘果多糖对提高正常小鼠的脾脏指数及免疫调节功能具有较好效果，但浓度过高会引起小鼠胸腺萎缩，此研究结果为桃金娘的进一步开发和利用提供参考依据。

Abstract:

To investigate the effect of polysaccharide immune function of healthy mice, Rhodomyrtus tomentosa fruit Polysaccharide was prepared and its monosaccharide composition was analyzed. The polysaccharide was prepared by water bath extraction, and the monosaccharide composition was analyzed by derivatization high performance liquid chromatography. 80 Kunming mice were randomly divided into normal group, astragalus polysaccharide group [intraperitoneal injection of 200 μg/(g·BW)] and Rhodomyrtus tomentosa fruit polysaccharide low, medium and high dose group [intraperitoneal injection of 50 μg / (g·BW), 100 μg / (g·BW) and 200 μg / (g·BW), respectively]. The mice in normal group were treated with normal saline of equal volume. This injection was administered once a day for 7 days. Then thymus index, spleen index, the content of IL-1β, IL-2, IL-6, IFN-γ, LZM and POD in serum were determined. The results showed that the polysaccharide content in Rhodomyrtus tomentosa fruit was 65.83 %, which was mainly composed of mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose. The thymus index of mice was reduced by Rhodomyrtus tomentosa fruit polysaccharide, and the spleen index was enhanced significantly. The levels of IL-1β, IL-2, IL-6 and IFN-γ in serum and the activity of LZM and POD were also increased by the polysaccharide. Rhodomyrtus tomentosa polysaccharide can actively promote the spleen index and immune regulation function of normal mice, but too high concentration can cause thymus atrophy in mice. The results can provide a reference for Rhodomyrtus tomentosa’s further development and utilization.