一株输入性猫传染性腹膜炎病毒的分离鉴定和遗传特征分析

Isolation and Characterization of a Feline Infectious Peritonitis Virus

关键词:  猫传染性腹膜炎病毒  分离鉴定  S基因  N基因  遗传进化分析

Key words:  feline infectious peritonitis virus  isolation and identification  S gene  N gene  genetic variation

摘要:

从北京地区临床疑似传染性腹膜炎患猫的粪便和腹水样品中分离猫传染性腹膜炎病毒(Feline infectious peritonitis virus, FIPV)，共采集10份样品进行RT-PCR检测，并接种MDCK细胞进行病毒分离和鉴定。结果表明：10份样品的RT-PCR检测结果均为FIPV阳性，将样品接种MDCK细胞并经连续传代培养，成功分离到一株FIPV，经鉴定属于FCoV-Ⅱ血清型，将其命名为FIPV/BJ01株；该毒株可以在MDCK细胞上增殖并产生典型的细胞病变，病毒滴度为105.5TCID50/0.1mL；S和N基因的核苷酸与其他毒株的同源性分别为63.3%~99.4%和45.5%~992%，均与美国毒株同源性较高，而与中国毒株的同源性较低。

Abstract:

In this study, feline infectious peritonitis virus (FIPV) was isolated from feces and ascites samples of cats with clinically suspected infectious peritonitis collected in Beijing. A total of 10 samples were collected for RT-PCR detection, and MDCK cells were inoculated for virus isolation and identification. The results showed that the RT-PCR results of 10 samples were FIPV positive. After inoculating the samples with MDCK cells and continuous subculture, a FIPV strain was successfully isolated. It was identified as FCoV-Ⅱ serotype and named FIPV/BJ01 strain; the strain could proliferate on MDCK cells and produce typical cytopathy. The virus content was 105.5(TCID50)/0.1mL; the nucleotide homology of S and N genes with other strains was 63.3%~99.4% and 45.5%~99.2% respectively. They both had high homology with American strains and low homology with Chinese strains.