| 摘要: |
| 评定液相色谱-串联质谱法（LC-MS/MS）测定猪肉中四环素类药物残留量的不确定度。依据GB/T 21317-2007《动物源性食品中四环素类兽药残留量检测方法 液相色谱-质谱/质谱法与高效液相色谱法》，建立相关数学模型，对实验过程中的不确定度来源进行分析与评定。当采用LC-MS/MS法测定猪肉中土霉素、四环素、金霉素、强力霉素的残留量分别为50.05、42.81、42.45、41.60μg/kg时，扩展不确定度分别为 5.44、6.72、2.86、4.80μg/kg (k=2)。检测结果的不确定度主要受回收率、标准溶液配制、工作曲线拟合的影响。 |
| 关键词: 四环素 不确定度 LC-MS/MS |
| DOI： |
| 投稿时间：2021-09-30修订日期：2022-03-04 |
| 基金项目:中央高校基本科研业务费（自然科学）（XDJK2020B062) |
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| Uncertainty Evaluation for The Determination of Tetracycline Drug Residues in Pig Muscle by Liquid Chromatography-Tandem Mass Spectrometry Method |
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| (Quality Control and Inspection Center for Domestic Animal Product, Ministry of Agriculture and Rural Affairs (Chongqing)) |
| Abstract: |
| To evaluate the uncertainty of the determination of tetracycline drug residues in pig muscle by Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS). According to GB/T 21317-2007 Determination of tetracycline residues in food of animal origin by high performance liquid chromatography-tandem mass spectrometry method and high performance liquid chromatography method, relevant mathematical models were established to analyze and evaluate the sources of uncertainty in the LC-MS/MS experiment. When the content of oxytetracycline, tetracycline, aureomycin, doxytetracycline in pig muscle was 50.05、42.81、42.45、41.60μg/kg by LC-MS/MS, its extended relative uncertainty was 5.44, 6.72, 2.86, and 4.80μg/kg (k=2), respectively. The uncertainty of detection results is mainly affected by recovery rate, standard solution preparation and working curve fitting. |
| Key words: Tetracycline drug Uncertainty LC-MS/MS |
