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硫酸头孢喹肟在安格斯牛体内药物代谢动力学研究 |
刘雪松,陈亮,冯万宇,张蕾,沈思思,兰世捷,李丹,苗艳,黄宝银,史同瑞 |
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(黑龙江省农业科学院畜牧兽医分院) |
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摘要: |
为了探究硫酸头孢喹肟在安格斯牛体内的药物代谢动力学,对安格斯牛以2 mg/kg剂量分别进行静脉、肌肉、皮下注射后,于不同时间点颈静脉采血,应用HPLC测定血液中硫酸头孢喹肟的药物浓度,计算药动学参数。静脉、肌肉、皮下注射的消除半衰期(T1/2β)分别为(2.05±0.49)h、(2.17±0.51)h、(2.37±0.47)h,达峰时间(Tmax)分别为(0.75±0.25)h、(0.87±0.25)h、(1±0.37)h,达峰浓度(Cmax)分别为(7.31±1.98)μg/mL、(5.34±1.21)μg/mL、(5.16±1.29)μg/mL,药时曲线下面积(AUC)分别为(24.72±5.31)μg.h/mL、(19.97±3.11)μg.h/mL、(20.51±4.87)μg.h/mL,平均留置时间(MRT)分别为(2.83±0.61)h、(3.02±0.71)h、(3.26±0.89)h,清除率(CL)分别为(0.07±0.02)L/h.kg、(0.09±0.03)L/h.kg、(0.09±0.04)L/h.kg,表观分布容积(Vd)分别为(0.23±0.06)L/kg、(0.31±0.08)L/kg、(0.32±0.09)L/kg。肌肉、皮下注射的绝对生物利用度(F)分别为80.78%、82.96%。表明硫酸头孢喹肟在安格斯牛体内吸收快、消除慢,肌肉注射以及皮下注射的绝对利用度高。 |
关键词: 硫酸头孢喹肟 高效液相色谱 药物代谢动力学 静脉注射 肌肉注射 皮下注射 |
DOI: |
投稿时间:2021-08-25修订日期:2021-09-23 |
基金项目:黑龙江省农业科学院“农业科技创新跨越工程”专项(HNK2019CX16) |
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Pharmacokinetics of cefquinome sulfate in Angus cattle |
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(Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences) |
Abstract: |
After intravenous, intramuscular and subcutaneous injection of cefquiome sulfate at the dose of 2 mg/kg, plasma samples of Angus cattle was collected from jugular vein at different time points. The concentration of cefquinome sulfate in plasma samples was determined by HPLC. The concentration-time profiling of cefquinome sulfate within the plasma specimens was analyzed by the non-compartmental analysis. The elimination-half-life(T1/2β) of intravenous, intramuscular and subcutaneous injection were (2.05±0.49) h, (2.17±0.51) h, (2.37±0.47) h, respectively. The time to achieve maximum concentration (Tmax) of intravenous, intramuscular and subcutaneous injection were (0.75±0.25) h, (0.87±0.25) h, (1±0.37) h, respectively. The maximum concentration in plasma (Cmax) of intravenous, intramuscular and subcutaneous injection were (7.31±1.98) μg/mL, (5.34±1.21) μg/mL, (5.16±1.29) μg/mL, respectively. The area under the concentration-time curve (AUC) of intravenous, intramuscular and subcutaneous injection were (24.72±5.31) μg.h/mL, (19.97±3.11) μg.h/mL, (20.51±4.87 ) μg.h/ mL, respectively. The mean residence time (MRT) of intravenous, intramuscular and subcutaneous injection were (2.83±0.61) h, (3.02±0.71) h and (3.26±0.89) h, respectively.The clearance (CL) of intravenous, intramuscular and subcutaneous injection were (0.07±0.02) L/h.kg, (0.09±0.03) L/h.kg and (0.09±0.04) L/h.kg, respectively. The apparent volume of distribution (Vd) of intravenous, intramuscular and subcutaneous injection were (0.23±0.06) L/kg, (0.31±0.08) L/kg and (0.32±0.09) L/kg, respectively. The absolute bioavailability (F) of intramuscular and subcutaneous injection were 80.78% and 82.96% respectively. The results showed that cefquinome sulfate has the characteristics of rapid absorption and slow elimination in Angus cattle. The absolute bioavailability of intramuscular and subcutaneous injection was high. |
Key words: cefquinome sulfate high performance liquid chromatography pharmacokinetics intravenous injection intramuscular injection subcutaneous injection |