鱼腥草多糖对MA104和MARC145细胞活力影响的比较研究

Effects of Houttuynia Cordata Polysaccharides on Viability of MA104 and MARC145 Cells

关键词:  鱼腥草多糖  MA104细胞  MARC145细胞  细胞活力  MTT法

Key words:  houttuynia cordata polysaccharide  MA104 cells  MARC145 cells  cell viability  MTT assay

摘要:

本试验研究鱼腥草多糖（houttuynia cordata polysaccharide, HCP）对MA104和MARC145细胞活力的影响，筛选梯度浓度的鱼腥草多糖对MA104和MARC145细胞促生长最佳浓度剂量范围和毒性剂量。通过水提醇沉法提取HCP，得糖率为8.18%；分别采用MTT法和细胞平板计数法绘制MA104和MARC145细胞的生长曲线；应用MTT法检测梯度浓度HCP对MA104和MARC145细胞活力的影响。结果表明，50 mg/mL HCP对细胞活力有抑制作用（P<0.05），0.05~5 mg/mL HCP对细胞活力有促进作用（P<0.05），0~0.005 mg/mL HCP对细胞活力无显著影响（P＞0.05）。结果提示，HCP（0~5 mg/mL）对MA104和MARC145细胞无毒性作用。

Abstract:

(Objective)The effects of Houttuynia cordata polysaccharide(HCP)on viability of MA104 and MARC145 cells were studied in this experiment. (Method)The sugar yield of HCP was 8.18% by water extraction and alcohol precipitation. The growth curves of MA104 and MARC145 cells were plotted by MTT method and cell counting method, respectively. MTT assay was used to detect the effects of gradient concentration of HCP on viability of MA104 and MARC145 cells. (Result)The results showed that viability of MA104 and MARC145 cells were inhibited by 50 mg/mL HCP, these were promoted by 0.05~5 mg/mL HCP, and these were no significant effect by 0~0.005 mg/mL HCP. (Conclusion)Conclusion: Below 5 mg/mL HCP had no toxic effect on MA104 and MARC145 cells.