基于AHP-CRITIC法的正交设计优选贞芪颗粒提取工艺及促生长作用初步研究

Studies on optimization of extraction process and preliminary growth-promoting activities of Zhenqi granules by orthogonal design based on AHP-CRITIC analysis

关键词:  贞芪颗粒  AHP-CRITIC混合加权法  正交试验  提取工艺  促生长

Key words:  Zhenqi granules  mixed weighted AHP-CRITIC  orthogonal test  extraction process  promote growth

摘要:

（目的）开发一种具有促生长作用的新中兽药贞芪颗粒，优化其提取工艺并对其促生猪生长作用进行初步研究，为其工业化生产提供参考。（方法）以总多糖、黄芪甲苷、毛蕊异黄酮葡萄糖苷、特女贞苷、红景天苷、干膏得率作为评价指标，采用层次分析法（AHP）、基于指标相关性的权重确定方法（CRITIC）、AHP-CRITIC混合加权法确定各评价指标的权重系数，根据其正交试验结果优化贞芪颗粒提取工艺参数。将100头保育猪随机分为5组，通过在日粮中添加不同剂量的颗粒，连续喂养30天，与空白组和阳性对照组对比，计算平均日增重（ADG）、平均日采食量（ADFI）和料肉比（F: G）。（结果）AHP-CRITIC混合加权法科学优选出最佳提取工艺参数为复方药材加12-13倍质量的水提取3次（第一次13倍），每次2.5 h。3批验证试验综合评分均值为98.91，RSD为0.79%。经过30天饲喂，高、中、低剂量组和阳性对照组的料重比无显著性差异（P>0.05），但都显著低于空白对照组（P<0.05），其中中剂量组的料重比最低。（结论）AHP-CRITIC混合加权法确定的权重系数客观、真实，优选出的贞芪颗粒提取工艺经验证，合理、稳定，重复性好，此工艺条件下制备的颗粒对生猪具有促进生长的作用。

Abstract:

(OBJECTIVE) To develop a new Chinese veterinary drug Zhenqi granules with growth- promoting effect, the optimizing of extraction process and preliminary evaluation of the growth-promoting activities of Zhenqi granules were conducted, providing reference for its industrial production. (METHODS) The extraction parameters of Zhenqi granules were optimized according to the orthogonal tests where the total amounts of polysaccharides, astragaloside IV, calycosin-7-O-β-D-glucoside, specnuezhenide, salidroside and the yield of dry paste were used as comprehensive evaluation indicators and their respective weighting coefficients were determined by analytic hierarchy process(AHP), criteria importance through intercriteria correlation(CRITIC) method and mixed weighted AHP-CRITIC. Then, 100 nursery pigs were randomly divided into 5 groups, which were fed continuously for 30 days by adding different doses of granules to the dietary supplementation. Finally, the average daily gain(ADG), average daily feed intake(ADFI) and feed to gain ratio(F:G) compared with blank group and positive control group were calculated. (RESULTS) The best extraction parameters were optimized by mixed weighted AHP-CRITIC method as following: the compound medicinal materials were extracted qualitatively with 12-13 times of water for thrice(13 times at first), and each turn for 2.5 h. The mean comprehensive score of the three batches of validation tests was 98.91, and the RSD was 0.79%. After feeding for 30 days, no significant difference in the F:G between the high, middle and low dose groups and the positive control group(P>0.05), but they were all significantly lower than the blank control group(P<0.05), and the F:G of the middle dose group was the lowest. (CONCLUSION) The weight coefficient determined by AHP-CRITIC is objective and true, and the optimized extraction parameters of Zhenqi granules have been verified to be reasonable, stable and reproducible. The granules produced under the optimized conditions can promote the growth of pigs, which can be used in industrial production.