中国山羊痘弱毒疫苗AV41株基因组分子标记的比较分析

Genomic Molecular Markers Comparative Analysis of Attenuated Goat Poxvirus Vaccine AV41 in China

关键词:  牛结节性皮肤病  山羊痘弱毒疫苗AV41 株  分子标记

Key words:  Lumpy skin disease  goatpox virus attenuated vaccine  molecular marker

摘要:

为鉴别我国山羊痘病毒（GTPV）弱毒疫苗AV41株与牛结节性皮肤病病毒（LSDV）野毒株，运用生物信息学方法将AV41株基因组全序列与GenBank中已发表的LSDV、GTPV和绵羊痘病毒（SPPV）基因组进行比较，筛选AV41株基因组分子标记。采用我国不同厂家来源的AV41株疫苗测序验证分子标记相关序列，并与我国LSDV野毒株相应位置序列比较。结果显示，通过基因组比较分析共筛选获得分子标记19 个，经测序确认其中7 个为AV41株基因组独特分子标记，包括DNA ligase基因2433A2434、Kelch-like protein基因1831A1832、GTPV_gp020基因G203A、GTPV_gp021基因C681T、DNA-binding viron core protein基因C47T、RNA polymerase subunit 基因T64C和Myristylated protein基因C746A，而我国LSDV野毒株相应位置序列未见上述变化。本研究揭示了AV41株基因组独特分子标记分布情况，可用于我国牛结节性皮肤病疫情防控中与LSDV野毒株的分子鉴别。

Abstract:

To distinguish Goatpox virus (GTPV) attenuated vaccine AV41 from wild-type Lumpy skin disease virus (WT-LSDV) in China, genomic molecular markers of AV41 were primitively analyzed using bioinformatics method from complete genomic comparative analysis of LSDV、GTPV and Sheeppox virus(SPPV) strains published in GenBank. Thereafter, sequences with relevant molecular markers were verified by sequencing AV41 from different manufacturers and comparing with corresponding positions of WT-LSDV genome. Nineteen molecular markers were successfully screened from genome of AV41. Seven of these markers were specific to AV41 after being further confirmed, including DNA ligase gene 2433A2434、Kelch-like protein gene 1831A1832、GTPV_gp020 gene G203A、GTPV_gp021 gene C681T、DNA-binding viron core protein gene C47T、RNA polymerase subunit gene T64C and Myristylated protein gene C746A, which were not observed in WT-LSDV. Systematically, this study implied the distribution of AV41 specific genomic molecular markers, which can be utilized for distinguishing AV41 from WT-LSDV during the epidemic prevention and monitor of Lumpy skin disease in China.